Schuierer, Marion and Hilger-Eversheim, Kristina and Dobner, Thomas and Bosserhoff, Anja-Katrin and Moser, Markus and Turner, Jeremy and Crossley, Merlin and Buettner, Reinhard (2001) Induction of AP-2 alpha expression by adenoviral infection involves inactivation of the AP-2rep transcriptional corepressor CtBP1. JOURNAL OF BIOLOGICAL CHEMISTRY, 276 (30). pp. 27944-27949. ISSN 0021-9258
Full text not available from this repository. (Request a copy)Abstract
AP-2 transcription factors execute important functions during embryonic development and malignant transformation. Recently, we have isolated a transcriptional repressor of AP-2 alpha expression, the novel Kruppel-related zinc finger protein AP-2rep (KIf12). Here, we show that repression of AP-2 alpha transcription by AP-2rep is dependent on an N-terminal PVDLS motif that interacts specifically with the corepressor CtBP1 both in vivo and in vitro. This interaction motif was previously identified in the C-terminal region of the adenoviral oncoprotein EIA. Infection of both HeLa and PA-1. cells with adenovirus type 5 strongly induced AP-2 alpha mRNA. Consistently, EIA was necessary and sufficient to mediate up-regulation of AP-2 alpha. Transiently transfected wild-type EIA protein activated an AP-2rep sensitive cis-regulatory element of the AP-2 alpha promoter, but E1A protein harboring a mutation in the PVDLS motif failed to activate. In summary, we conclude that the adenoviral oncoprotein EIA activates transcription from the endogenous AP-2 alpha gene, an effect that involves transcriptional derepression of the AP-2 alpha promoter by interaction of E1A with the AP-2rep corepressor CtBP1.
| Item Type: | Article |
|---|---|
| Uncontrolled Keywords: | TERMINAL BINDING-PROTEIN; REGION 1A PROTEINS; GENE-EXPRESSION; GENOMIC STRUCTURE; CELLS; E1A; REPRESSOR; AP-2-BETA; CLONING; DNA; |
| Subjects: | 600 Technology > 610 Medical sciences Medicine |
| Divisions: | Medicine > Lehrstuhl für Medizinische Mikrobiologie und Hygiene |
| Depositing User: | Dr. Gernot Deinzer |
| Date Deposited: | 21 Dec 2021 06:15 |
| Last Modified: | 21 Dec 2021 06:15 |
| URI: | https://pred.uni-regensburg.de/id/eprint/41252 |
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