Eder, Wolfgang and Jahnke, Linda L. and Schmidt, Mark and Huber, Robert (2001) Microbial diversity of the brine-seawater interface of the Kebrit Deep, Red Sea, studied via 16S rRNA gene sequences and cultivation methods. APPLIED AND ENVIRONMENTAL MICROBIOLOGY, 67 (7). pp. 3077-3085. ISSN 0099-2240
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The brine-seawater interface of the Kebrit Deep, northern Red Sea, was investigated for the presence of microorganisms using phylogenetic analysis combined with cultivation methods. Under strictly anaerobic culture conditions, novel halophiles were isolated. The ne,v rod-shaped isolates belong to the halophilic genus Halanaerobium and are the first representatives of the genus obtained from deep-sea, anaerobic brine pools. Within the genus Halanaerobium, they represent new species which grow chemoorganotrophically at NaCl concentrations ranging from 5 to 34%. The cellular fatty acid compositions are consistent with those of other Halanaerobium representatives, showing unusually large amounts of Delta7 and Delta 11 16:1 fatty acids. Phylogenetic analysis of the brine-seawater interface sample revealed the presence of various bacterial 16S rRNA gene sequences dominated by cultivated members of the bacterial domain, with the majority affiliated with the genus Halanaerobium. The new Halanaerobium 16S rRNA clone sequences showed the highest similarity (99.9%) to the sequence of isolate KT-8-13 from the Kebrit Deep brine. In this initial survey, our polyphasic approach demonstrates that novel halophiles thrive in the anaerobic, deep-sea brine pool of the Kebrit Deep, Red Sea. They may contribute significantly to the anaerobic degradation of organic matter enriched at the brine-seawater interface.
| Item Type: | Article |
|---|---|
| Uncontrolled Keywords: | FATTY-ACID-COMPOSITION; GREAT-SALT-LAKE; ORCA BASIN; SP-NOV; ATLANTIS-II; ANAEROBIC BACTERIUM; REDUCING BACTERIUM; HYPERSALINE BASIN; METHYL STEROL; SP. NOV.; |
| Subjects: | 500 Science > 570 Life sciences |
| Divisions: | Biology, Preclinical Medicine > Institut für Biochemie, Genetik und Mikrobiologie > Lehrstuhl für Mikrobiologie (Archaeenzentrum) |
| Depositing User: | Dr. Gernot Deinzer |
| Date Deposited: | 21 Dec 2021 08:06 |
| Last Modified: | 21 Dec 2021 08:06 |
| URI: | https://pred.uni-regensburg.de/id/eprint/41269 |
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