Wolf, Konrad and Castrop, Hayo and Riegger, Guenter A. J. and Kurtz, Armin and Kraemer, Bernhard K. (2001) Differential gene regulation of renal salt entry pathways by salt load in the distal nephron of the rat. PFLUGERS ARCHIV-EUROPEAN JOURNAL OF PHYSIOLOGY, 442 (4). pp. 498-504. ISSN 0031-6768
Full text not available from this repository. (Request a copy)Abstract
The aim of the present study was to determine the molecular responses of the main salt-reabsorbing systems in the distal nephron to changes of salt load of the organism. For this purpose we analysed messenger ribonucleic acid (mRNA) levels for the bumetanide-sensitive Na(+)K(+)2Cl(-) cotransporter (BSCl), the thiazide-sensitive Na+Cl- cotransporter (TSC), the kidney-specific inwards rectifier K+ channel (ROMK), the amiloride-sensitive epithelial Na+ channel (ENaC) and the kidney-specific Cl- channel ClC-K2, in the cortex and inner and outer medulla of kidneys from male Sprague-Dawley rats fed a high- (8% w/w), normal- (0.6%) or low(0.02%) salt diet or treated chronically with subcutaneous infusions of furosemide (12 mg/kg per day). BSCl and ROMK mRNA levels did not differ between the four treatment groups. TSC mRNA increased during furosemide treatment 1.75-fold versus control but was not affected by a high- or a low-salt diet. The mRNA for the a-subunit of ENaC increased with the low-salt diet (about 1.5-fold) and with furosemide (about 2.1-fold) in all kidney zones, but did not change with the high-salt diet. Dietary salt loading down-regulated ClC-K2 mRNA in the outer medulla 0.6-fold versus control whilst furosemide treatment, but not the low-salt diet, increased ClC-K2 mRNA in the outer (1.6-fold) and inner medulla (2.0-fold). These findings suggest that gene expression of Na+ and Cl- entry pathways in the distal nephron are at least partly regulated by the salt load of the organism, such that salt-reabsorbing systems are stimulated by salt deficiency and suppressed by salt overload.
| Item Type: | Article |
|---|---|
| Uncontrolled Keywords: | ALDOSTERONE-INDUCED PROTEIN; THICK ASCENDING LIMB; NA-CL COTRANSPORTER; BARTTERS-SYNDROME; MESSENGER-RNA; CHANNEL; KIDNEY; MUTATIONS; LOCALIZATION; EXPRESSION; chloride channel; gene transcription; kidney; salt diet; sodium balance |
| Subjects: | 500 Science > 570 Life sciences 600 Technology > 610 Medical sciences Medicine |
| Divisions: | Medicine > Lehrstuhl für Innere Medizin II Biology, Preclinical Medicine > Institut für Physiologie > Prof. Dr. Armin Kurtz Biology, Preclinical Medicine > Institut für Physiologie > Prof. Dr. Wolf Hayo Castrop |
| Depositing User: | Dr. Gernot Deinzer |
| Date Deposited: | 12 Jan 2022 13:43 |
| Last Modified: | 12 Jan 2022 13:43 |
| URI: | https://pred.uni-regensburg.de/id/eprint/41317 |
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