Raab, Ulla and Bauer, Birgit and Gigler, Andreas and Beckenlehner, Karin and Wolf, Hans and Modrow, Susanne (2001) Cellular transcription factors that interact with p6 promoter elements of parvovirus B19. JOURNAL OF GENERAL VIROLOGY, 82. pp. 1473-1480. ISSN 0022-1317, 1465-2099
Full text not available from this repository. (Request a copy)Abstract
All transcripts of the human parvovirus B19 identified so far are regulated by a single promoter at map unit 6 of the viral genome, the so-called p6 promoter. This promoter is active in a wide variety of different cells. In order to identify cellular transcription factors involved in regulating promoter activity, we performed gel-retardation and supershift assays using the parts of the p6 promoter sequence shown previously to be protected in footprint experiments. Thereby, binding was demonstrated of the Oct-1 protein to an octamer motif within the p6 promoter and of the transcription factor Sp1 to three GC boxes. A specific preferential interaction of the factor Sp3 with one of these boxes was observed, indicating that the ratio Sp1:Sp3 may be involved in the regulation of promoter activity, Consensus sites for the regulatory protein YY1 are located close to the GC boxes and the octamer motif, to which this factor binds efficiently.
| Item Type: | Article |
|---|---|
| Uncontrolled Keywords: | GENE-EXPRESSION; REPRESS TRANSCRIPTION; UTEROGLOBIN PROMOTER; FACTORS SP1; PROTEIN; CLONING; FAMILY; CELLS; BOX; YY1; |
| Subjects: | 600 Technology > 610 Medical sciences Medicine |
| Divisions: | Medicine > Lehrstuhl für Medizinische Mikrobiologie und Hygiene |
| Depositing User: | Dr. Gernot Deinzer |
| Date Deposited: | 24 Jan 2022 13:44 |
| Last Modified: | 24 Jan 2022 13:44 |
| URI: | https://pred.uni-regensburg.de/id/eprint/41395 |
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