Buechler, Christa and Ritter, Mirko and Duong, Chinh Quoc and Orso, Evelyn and Kapinsky, Michael and Schmitz, Gerd (2001) Adipophilin is a sensitive marker for lipid loading in human blood monocytes. BIOCHIMICA ET BIOPHYSICA ACTA-MOLECULAR AND CELL BIOLOGY OF LIPIDS, 1532 (1-2). pp. 97-104. ISSN 1388-1981
Full text not available from this repository. (Request a copy)Abstract
Adipophilin, a marker of lipid accumulation initially described in adipocytes, was recently shown to be induced in macrophage roam cells. We found that even freshly isolated blood monocytes express adipophilin and that the amount of adipophilin protein is variable in monocytes from different healthy individuals. However, the physiological expression of adipophilin does not correlate with the levels of free fatty acids, cholesterylesters or free cholesterol. Enzymatically modified low-density lipoprotein (E-LDL) induces rapid foam cell formation in monocytes and upregulates adipophilin mRNA and protein within 2 h of incubation. This rapid induction of adipophilin is accompanied by a significant increase of free fatty acids in monocytes incubated with E-LDL. Adipophilin facilitates the uptake of free fatty acids, and here we demonstrate that free fatty acids increase is related to the early upregulation of adipophilin expression in blood monocytes. Fatty acids are ligands for peroxisome proliferator-activated receptor-gamma (PPAR gamma), and the upregulation of adipophilin mRNA by PPAR gamma agonists like 15d-PGJ(2) and ciglitazone indicates that PPAR gamma may mediate the induction of adipophilin expression in human blood monocytes. (C) 2001 Elsevier Science B.V. All rights reserved.
| Item Type: | Article |
|---|---|
| Uncontrolled Keywords: | DIFFERENTIATION-RELATED PROTEIN; LOW-DENSITY-LIPOPROTEIN; PPAR-GAMMA AGONISTS; OXIDIZED LDL; EXPRESSION; ATHEROGENESIS; STIMULATION; MACROPHAGES; COMPLEMENT; ACTIVATION; blood monocyte; adipophilin; enzymatically modified low density lipoprotein; proliferator activated receptor gamma; free fatty acid |
| Subjects: | 600 Technology > 610 Medical sciences Medicine |
| Divisions: | Medicine > Lehrstuhl für Klinische Chemie und Laboratoriumsmedizin |
| Depositing User: | Dr. Gernot Deinzer |
| Date Deposited: | 25 Jan 2022 15:35 |
| Last Modified: | 25 Jan 2022 15:35 |
| URI: | https://pred.uni-regensburg.de/id/eprint/41423 |
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