Huber, Christian and Klimant, Ingo and Krause, Christian and Wolfbeis, Otto S. (2001) Dual lifetime referencing as applied to a chloride optical sensor. ANALYTICAL CHEMISTRY, 73 (9). pp. 2097-2103. ISSN 0003-2700
Full text not available from this repository. (Request a copy)Abstract
A membrane with an optical response to chloride has been developed that contains two luminophores that display two largely different decay times. The first luminophore (the "reference") is a chloride-insensitive ruthenium metal-ligand complex possessing a decay time in the microsecond range. The second luminophore is the short-lived chloride-quenchable fluorescent probe lucigenin. Both are contained in a hydrogel matrix and are excited by a blue LED emitting sinusoidally modulated light. Under these conditions, the chloride-dependent fluorescence intensity of lucigenin can be converted in an analyte-dependent fluorescence phase shift that depends on the ratio of the two luminescence intensities and can be measured at modulation frequencies of typically 45 kHz. The dynamic range of this sensor can be adjusted by either varying the ratio of the two luminophores dr selecting a particular optical filter combination.
| Item Type: | Article |
|---|---|
| Uncontrolled Keywords: | SOLVENT-POLYMERIC MEMBRANE; COEXTRACTION; COMPLEXES; FILMS; PH; |
| Subjects: | 500 Science > 540 Chemistry & allied sciences |
| Divisions: | Chemistry and Pharmacy > Institut für Analytische Chemie, Chemo- und Biosensorik > Chemo- und Biosensorik (Prof. Antje J. Bäumner, formerly Prof. Wolfbeis) |
| Depositing User: | Dr. Gernot Deinzer |
| Date Deposited: | 31 Jan 2022 10:19 |
| Last Modified: | 31 Jan 2022 10:19 |
| URI: | https://pred.uni-regensburg.de/id/eprint/41442 |
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