Reischl, Udo and Lehn, Norbert and Sanden, Gary N. and Loeffelholz, Mike J. (2001) Real-time PCR assay targeting IS481 of Bordetella pertussis and molecular basis for detecting Bordetella holmesii. JOURNAL OF CLINICAL MICROBIOLOGY, 39 (5). pp. 1963-1966. ISSN 0095-1137
Full text not available from this repository. (Request a copy)Abstract
Detection of Bordetella holmesii by a real-time PCR assay targeting IS481 of Bordetella pertussis is reported. Sequencing of IS481-specific PCR products from B. pertussis and B. holmesii isolates revealed sequence homology. Restriction fragment length polymorphism demonstrated a low copy number of IS481-like sequences in B. holmesii. These results, and culture of B. holmesii from patients with cough, suggest that the specificity and predictive value of IS481-based PCR assays for pertussis may be compromised.
| Item Type: | Article |
|---|---|
| Uncontrolled Keywords: | POLYMERASE CHAIN-REACTION; MYCOBACTERIUM-TUBERCULOSIS; DNA POLYMORPHISM; PARAPERTUSSIS; INFECTIONS; DIAGNOSIS; INSERTION; SEQUENCE; STRAINS; IDENTIFICATION; |
| Subjects: | 600 Technology > 610 Medical sciences Medicine |
| Divisions: | Medicine > Lehrstuhl für Medizinische Mikrobiologie und Hygiene |
| Depositing User: | Dr. Gernot Deinzer |
| Date Deposited: | 31 Jan 2022 15:08 |
| Last Modified: | 31 Jan 2022 15:08 |
| URI: | https://pred.uni-regensburg.de/id/eprint/41461 |
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