Wolf, Konrad and Kurtz, Armin and Pfeifer, Michael and Hoecherl, Klaus and Riegger, Guenter A. J. and Kraemer, Bernhard K. (2001) Different regulation of left ventricular ANP, BNP and adrenomedullin mRNA in the two-kidney, one-clip model of renovascular hypertension. PFLUGERS ARCHIV-EUROPEAN JOURNAL OF PHYSIOLOGY, 442 (2). pp. 212-217. ISSN 0031-6768
Full text not available from this repository. (Request a copy)Abstract
The aim of our study was to clarify whether atrial (ANP and brain (BNP) natriuretic peptides and the hypotensive peptide adrenomedullin (ADM) are regulated differently in the rat heart in the two-kidney, one-clip model of renovascular hypertension. We assessed messenger ribonucleic acid (mRNA) abundance and distribution of ANP, BNP and ADM in the ventricles and atria of rats after unilateral renal artery stenosis (clipping). Rats were clipped for 6 h or 1, 2 or 4 days and mRNA levels were assessed semiquantitatively in left and right atria and ventricles by RNase protection assay. Left ventricular BNP mRNA up-regulation (4.3-fold after 6 hours) preceded ANP up-regulation (4.5-fold after 1 day) and seemed to be transient, whereas ANP mRNA levels were still elevated at day 4 (2.4-fold vs. sham). The right ventricle and the atria did not participate in these responses. Despite the massive changes of natriuretic peptide mRNAs, ADM mRNA did not change in either the ventricles or the atria. In contrast to ANP and BNP mRNA, which predominate in atrial tissue, mRNA for adrenomedullin is equally distributed in ventricles and atria. Plasma levels of immunoreactive (ir)-ANP and ir-BNP changed in parallel with left ventricular mRNA levels. Our findings suggest that renovascular hypertension induced by clipping the renal artery leads to immediate, but independent, up-regulation of ANP and BNP mRNA in the left ventricle whereas adrenomedullin mRNA is not changed.
| Item Type: | Article |
|---|---|
| Uncontrolled Keywords: | PEPTIDE GENE-EXPRESSION; BRAIN NATRIURETIC PEPTIDE; HYPOTENSIVE PEPTIDE; RESPONSE GENE; MESSENGER-RNA; RAT-HEART; IN-VITRO; ATRIAL; MYOCYTES; OVERLOAD; hypertension; kidney; natriuretic peptide; hypertrophy; adrenomedullin |
| Subjects: | 500 Science > 570 Life sciences 600 Technology > 610 Medical sciences Medicine 600 Technology > 615 Pharmacy |
| Divisions: | Medicine > Lehrstuhl für Innere Medizin II Biology, Preclinical Medicine > Institut für Physiologie > Prof. Dr. Armin Kurtz Chemistry and Pharmacy > Institute of Pharmacy > Pharmacology and Toxicology (Prof. Schlossmann, formerly Prof. Seifert) |
| Depositing User: | Dr. Gernot Deinzer |
| Date Deposited: | 01 Feb 2022 07:53 |
| Last Modified: | 01 Feb 2022 07:53 |
| URI: | https://pred.uni-regensburg.de/id/eprint/41475 |
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