Rath, Heiko C. and Schultz, Michael and Freitag, Rene and Dieleman, Levinus A. and Li, Fengling and Linde, Hans-Joerg and Schoelmerich, Juergen and Sartor, R. Balfour (2001) Different subsets of enteric bacteria induce and perpetuate experimental colitis in rats and mice. INFECTION AND IMMUNITY, 69 (4). pp. 2277-2285. ISSN 0019-9567, 1098-5522
Full text not available from this repository. (Request a copy)Abstract
Resident bacteria are incriminated in the pathogenesis of experimental colitis and inflammatory bowel diseases. We investigated the relative roles of various enteric bacteria populations in the induction and perpetuation of experimental colitis. HLA-B27 transgenic rats received antibiotics (ciprofloxacin, metronidazole, or vancomycin-imipenem) in drinking water or water alone in either prevention or treatment protocols. Mice were treated similarly with metronidazole or vancomycin-imipenem before or after receiving 5% dextran sodium sulfate (DSS). Germfree transgenic rats were colonized with specific-pathogen-free enteric bacteria grown overnight either in anaerobic or aerobic atmospheres. Nontransgenic rats colonized with anaerobic bacteria served as negative controls. Although preventive metronidazole significantly attenuated colitis in transgenic rats and DSS-treated mice, it had no therapeutic benefit once colitis was established. Ciprofloxacin also partially prevented but did not treat colitis in B27 transgenic rats. In both animal models vancomycin-imipenem most effectively prevented and treated colitis. Germfree transgenic rats reconstituted with enteric bacteria grown under anaerobic conditions had more aggressive colitis than those associated with aerobic bacteria. These results suggest that a subset of resident luminal bacteria induces colitis, but that a complex interaction of commensal aerobic and anaerobic bacteria provides the constant antigenic drive for chronic immune-mediated colonic inflammation.
| Item Type: | Article |
|---|---|
| Uncontrolled Keywords: | DEXTRAN SULFATE SODIUM; HLA-B27 TRANSGENIC RATS; EXPERIMENTAL ULCERATIVE-COLITIS; CHRONIC INTESTINAL INFLAMMATION; ACTIVE CROHNS-DISEASE; CONTROLLED TRIAL; INTERLEUKIN-10-DEFICIENT MICE; IMMUNODEFICIENT MICE; IL-2-DEFICIENT MICE; DEFICIENT MICE; |
| Subjects: | 600 Technology > 610 Medical sciences Medicine |
| Divisions: | Medicine > Lehrstuhl für Innere Medizin I Medicine > Lehrstuhl für Medizinische Mikrobiologie und Hygiene |
| Depositing User: | Dr. Gernot Deinzer |
| Date Deposited: | 07 Feb 2022 10:16 |
| Last Modified: | 07 Feb 2022 10:16 |
| URI: | https://pred.uni-regensburg.de/id/eprint/41556 |
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