Schuster, Uta and Schmalz, Gottfried and Thonemann, Birger and Mendel, Nadim and Metzl, Carola (2001) Cytotoxicity testing with three-dimensional cultures of transfected pulp-derived cells. JOURNAL OF ENDODONTICS, 27 (4). pp. 259-265. ISSN 0099-2399
Full text not available from this repository.Abstract
SV40 large T-antigen-transfected bovine pulp-derived cells were grown three-dimensionally on polyamide meshes. For optimal cell growth, various cell numbers and mesh coatings were tested. Next the three-dimensional cultures were used in a dentin barrier test device, and the system was evaluated by testing a set of dental filling materials. After 24 hr exposure with or without perfusion of the pulpal part of the test device, cell survival was evaluated using an WITT assay. In all experiments pulp-derived cells transfected with SV40 large T-antigen grew three-dimensionally on polyamide meshes and showed growth kinetics similar to those on cell culture plates with lag, log, and plateau phases (reached after about 14 days of incubation), Cross-sections of the three-dimensional cell cultures revealed about 15 to 20 cell layers. In vitro cytotoxicity tests resulted in cell survival rates which are in good agreement with in vivo data and with results obtained from cytotoxicity tests with three-dimensional cultures of human foreskin fibroblasts.
| Item Type: | Article |
|---|---|
| Uncontrolled Keywords: | FIBROBLASTS; INVITRO; MODEL; |
| Subjects: | 600 Technology > 610 Medical sciences Medicine |
| Divisions: | Medicine > Lehrstuhl für Zahnerhaltung und Parodontologie |
| Depositing User: | Dr. Gernot Deinzer |
| Date Deposited: | 07 Feb 2022 10:32 |
| Last Modified: | 07 Feb 2022 10:32 |
| URI: | https://pred.uni-regensburg.de/id/eprint/41587 |
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