Rozdzinski, Eva and Marre, Reinhard and Susa, Milorad and Wirth, Reinhard and Muscholl-Silberhorn, Albrecht (2001) Aggregation substance-mediated adherence of Enterococcus faecalis to immobilized extracellular matrix proteins. MICROBIAL PATHOGENESIS, 30 (4). pp. 211-220. ISSN 0882-4010
Full text not available from this repository. (Request a copy)Abstract
Aggregation substance (AS) of Enterococcus faecalis (E. faecalis), a sex pheromone plasmid encoded cell surface protein, mediates the formation of bacterial aggregates, thereby promoting plasmid transfer. The influence of pAD1-encoded AS, Asa1, on binding to immobilized extracellular matrix proteins was studied. The presence of AS increased enterococcal adherence to fibronectin more than eight-fold, to thrombospondin more than four-fold, to vitronectin more than three-fold, and to collagen type I more than two-fold (P < 0.001). In contrast, binding to laminin and collagen type IV occurred independently of AS. Adherence of the constitutively AS expressing E. faecalis OG1X(pAM721) to immobilized fibronectin was found to be approximately five times higher than that of Staphylococcus aureus Cowan and approximately 30 times higher than that of Streptococcus bovis. Investigation of strains with various deletions within the structural gene of asa1 suggests that attachment to immobilized fibronectin is mainly mediated by amino acids within the variable region or by neighbouring residues. Thus, AS may promote adherence to injured epithelium and endothelium, where extracellular matrix proteins are exposed, thereby facilitating colonization and infection. (C) 2001 Academic Press.
| Item Type: | Article |
|---|---|
| Uncontrolled Keywords: | HUMAN-ENDOTHELIAL-CELLS; FIBRONECTIN-BINDING PROTEIN; INTESTINAL EPITHELIAL-CELLS; STAPHYLOCOCCUS-AUREUS; SEX-PHEROMONE; STREPTOCOCCUS-FAECALIS; EXPERIMENTAL ENDOCARDITIS; ESCHERICHIA-COLI; PLASMID TRANSFER; THROMBOSPONDIN; Enterococcus faecalis; aggregation substance; extracellular matrix proteins |
| Subjects: | 500 Science > 570 Life sciences |
| Divisions: | Biology, Preclinical Medicine > Institut für Biochemie, Genetik und Mikrobiologie > Lehrstuhl für Mikrobiologie (Archaeenzentrum) > Prof. Dr. Reinhard Wirth |
| Depositing User: | Dr. Gernot Deinzer |
| Date Deposited: | 08 Feb 2022 06:57 |
| Last Modified: | 08 Feb 2022 06:57 |
| URI: | https://pred.uni-regensburg.de/id/eprint/41603 |
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