Grossmann, Johannes and Walther, K. and Artinger, M. and Kiessling, S. and Schoelmerich, Juergen (2001) Apoptotic signaling during initiation of detachment-induced apoptosis ("anoikis") of primary human intestinal epithelial cells. CELL GROWTH & DIFFERENTIATION, 12 (3). pp. 147-155. ISSN 1044-9523
Full text not available from this repository. (Request a copy)Abstract
Apoptosis after the loss of cell anchorage-"anoikis"-plays an important role in the life cycle of adherent cells. Furthermore, loss of anchorage dependency is believed to be a critical step in metastatic transformation. The aim of this study was to further characterize the sequence of intracellular events during anoikis in a nontransformed population of human intestinal epithelial cells (IECs). Purified human IECs were kept in suspension to induce anoikis in over 90% of IECs within 3 h. Two initiator caspases, caspase-2 and -9, are activated within 15 min, followed by the hierarchical activation of downstream caspases within 1 h. The activation of the caspase FLICE (caspase-8) does not contribute to the initiation of anoikis, and massive release of cytochrome c from mitochondria cannot be detected before 60 min, indicating that cytochrome c release does not play a role during initiation of anoikis. This study delineates the signaling cascade during anoikis of nontransformed cells, Future studies may identify alterations of this cascade in neoplastic cells, thereby possibly gaining insight into carcinogenesis and metastatic transformation.
| Item Type: | Article |
|---|---|
| Uncontrolled Keywords: | FOCAL ADHESION KINASE; PHOSPHOINOSITIDE 3-OH KINASE; CASPASE-MEDIATED CLEAVAGE; EXTRACELLULAR-MATRIX; CYTOCHROME-C; DNA FRAGMENTATION; PROTEIN-KINASE; DEATH; ACTIVATION; EXPRESSION; |
| Subjects: | 600 Technology > 610 Medical sciences Medicine |
| Divisions: | Medicine > Lehrstuhl für Innere Medizin I |
| Depositing User: | Dr. Gernot Deinzer |
| Date Deposited: | 14 Feb 2022 06:23 |
| Last Modified: | 14 Feb 2022 06:23 |
| URI: | https://pred.uni-regensburg.de/id/eprint/41682 |
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