Buechler, Christa and Ullrich, Heidrun and Ritter, Mirko and Porsch-Oezcueruemez, Mustafa and Lackner, Karl J. and Barlage, Stefan and Friedrich, Sven Olaf and Kostner, Gert M. and Schmitz, Gerd (2001) Lipoprotein (a) up-regulates the expression of the plasminogen activator inhibitor 2 in human blood monocytes. BLOOD, 97 (4). pp. 981-986. ISSN 0006-4971
Full text not available from this repository. (Request a copy)Abstract
Elevated plasma lipoprotein (a) (Lp[a]) and cardiac events show a modest but significant association in various clinical studies. However, the influence of high L(a) on the gene expression in blood monocytes as a major cell involved in atherogenesis is poorly described. To identify genes influenced by elevated serum Lp(a), the gene expression was analyzed on a complementary DNA microarray comparing monocytes from a patient with isolated Lp(a) hyperlipidemia and coronary heart disease with monocytes from a healthy blood donor with low Lp(a), By using this approach, numerous genes were found differentially expressed in patient-versus-control monocytes, Verification of these candidates by Northern blot analysis or semiquantitative polymerase chain reaction in monocytes from additional patients with Lp(a) hyperlipidemia and healthy blood donors with elevated Lp(a) confirmed a significant induction of plasminogen activator inhibitor type 2 (PAI-2) messenger RNA (mRNA) in monocytes from male, but not from female, individuals with high Lp(a), indicating that this observation is gender specific. This led also to increased intracellular and secreted PAI-2 protein in monocytes from male probands with Lp(a) hyperlipidemia. Plasminogen activator inhibitor type 1 (PAI-1) mRNA was found suppressed only in the patients' monocytes and not in healthy probands with high Lp(a) levels. Purified Lp(a) induced PAI-2 mRNA and protein and reduced PAI-1 expression in monocytes isolated from various controls. The finding that PAI-2 is elevated in monocytes from male patients with isolated Lp(a) hyperlipidemia and male healthy probands with high Lp(a) and that purified Lp(a) up-regulates PAI-2 in control monocytes in vitro indicate a direct, but gender-specific effect of Lp(a) for the induction of PAI-2 expression. (C) 2001 by The American Society of Hematology
| Item Type: | Article |
|---|---|
| Uncontrolled Keywords: | CELL-ADHESION; ENDOTHELIAL-CELLS; GENERATION; UROKINASE; LP(A); ACID; OVEREXPRESSION; PROTEOLYSIS; MECHANISM; DISEASE; |
| Subjects: | 600 Technology > 610 Medical sciences Medicine |
| Divisions: | Medicine > Lehrstuhl für Klinische Chemie und Laboratoriumsmedizin |
| Depositing User: | Dr. Gernot Deinzer |
| Date Deposited: | 15 Feb 2022 06:35 |
| Last Modified: | 15 Feb 2022 06:35 |
| URI: | https://pred.uni-regensburg.de/id/eprint/41750 |
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