Schmalz, Gottfried and Schuster, Uta and Thonemann, Birger and Barth, Mario and Esterbauer, Sandra (2001) Dentin barrier test with transfected bovine pulp-derived cells. JOURNAL OF ENDODONTICS, 27 (2). pp. 96-102. ISSN 0099-2399
Full text not available from this repository. (Request a copy)Abstract
Growth kinetics of SV40 large T-antigen-transfected bovine pulp-derived cells on dentin were investigated. These cells were used in a dentin barrier test device, and the system was evaluated by testing a set of dental filling materials. Cells (120 cells/mm(2)) were seeded on dentin slices and incubated for up to 21 days. Cell proliferation was recorded using WITT assay. For cytotoxicity tests 3500 cells/mm(2) were seeded on dentin discs, which were then incorporated into the dentin barrier test device. After 72 h preincubation test materials were applied. After a 24 h exposure with or without perfusion of the pulpal part of the test device, cell survival was evaluated using MTT assay. The cells revealed similar growth kinetics on dentin slices and on tissue culture plates. In cytotoxicity tests the cells were more sensitive toward the test materials than previously used three-dimensional cultures of human foreskin fibroblasts and as anticipated from clinical experience. Further improvement is expected by using three-dimensional cultures of pulp-derived cells.
| Item Type: | Article |
|---|---|
| Uncontrolled Keywords: | FIBROBLASTS; TOXICITY; INVITRO; |
| Subjects: | 600 Technology > 610 Medical sciences Medicine |
| Divisions: | Medicine > Lehrstuhl für Zahnerhaltung und Parodontologie |
| Depositing User: | Dr. Gernot Deinzer |
| Date Deposited: | 15 Feb 2022 09:58 |
| Last Modified: | 15 Feb 2022 09:58 |
| URI: | https://pred.uni-regensburg.de/id/eprint/41778 |
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