Robl, Ingrid and Grassl, Renate and Tanner, Widmar and Opekarova, Miroslava (2001) Construction of phosphatidylethanolamine-less strain of Saccharomyces cerevisiae. Effect on amino acid transport. YEAST, 18 (3). pp. 251-260. ISSN 0749-503X, 1097-0061
Full text not available from this repository. (Request a copy)Abstract
A triple yeast mutant was constructed which lacks BST1, the gene for sphingosine lyase, besides the phosphatidylserine decarboxylases PSD1 and PSD2. In this yeast mutant, which can only be grown in the presence of exogenous ethanolamine, phosphatidylethanolamine can be depleted to very low levels. Under those conditions, respiration as well as glucose and 3-O-methylglucose uptake proceed unaffected. Plasma membrane ATPase is as active in these cells as that of control cells grown in the presence of ethanolamine. Drastically decreased, however, are H+/amino acid symporters. The activities of arginine (Can1p), proline (Put4p) and general amino acid permease (Gap1p) are decreased more than 20-fold. Amino acid transport in yeast is dependent on coupling to the proton motive force. It can be envisaged that phosphatidylethanolamine might play a role in this process or in the early steps of the secretion pathway common for all amino acid permeases or, eventually, it could affect the transport proteins directly at the plasma membrane. Transformation of the triple mutant with a CEN plasmid harbouring BST1 wild-type gene totally reversed its phenotype to that observed in the double mutant, Copyright (C) 2000 John Wiley & Sons, Ltd.
| Item Type: | Article |
|---|---|
| Uncontrolled Keywords: | THIN-LAYER CHROMATOGRAPHY; ESCHERICHIA-COLI; PHOSPHATIDYLSERINE DECARBOXYLASE; LACTOSE PERMEASE; YEAST; PROTEIN; LIPIDS; GENE; PHOSPHOLIPIDS; REQUIREMENT; phosphatidylethanolamine; amino acid transport; Saccharomyces cerevisiae |
| Subjects: | 500 Science > 580 Botanical sciences |
| Divisions: | Biology, Preclinical Medicine > Institut für Pflanzenwissenschaften > Lehrstuhl für Zellbiologie und Pflanzenphysiologie (Prof. Dr. Klaus Grasser) |
| Depositing User: | Dr. Gernot Deinzer |
| Date Deposited: | 15 Feb 2022 13:48 |
| Last Modified: | 15 Feb 2022 13:48 |
| URI: | https://pred.uni-regensburg.de/id/eprint/41801 |
Actions (login required)
 |
View Item |
