Buonaguro, L. and Buonaguro, F. M. and Tornesello, M. L. and Mantas, D. and Beth-Giraldo, E. and Wagner, R. and Michelson, S. and Prevost, M. C. and Wolf, H. and Giraldo, G. (2001) High efficient production of Pr55(gag) virus-like particles expressing multiple HIV-1 epitopes, including a gp120 protein derived from an Ugandan HIV-1 isolate of subtype A. ANTIVIRAL RESEARCH, 49 (1). pp. 35-47. ISSN 0166-3542, 1872-9096
Full text not available from this repository.Abstract
The main goal of this study was to investigate a novel approach for an efficient and reproducible production of Virus-Like Particles (VLPs) expressing multiple HIV-1 epitopes. The HIV-1 Pr55(gag)-based VLPs have been produced in a Baculovirus expression system, using a transfer vector able to support the independent expression of different open reading frames (ORFs). In this regard, the gp120 derived from 94UG018 HIV-1(A) isolate, previously studied in our laboratory, has been packaged into the VLPs together with nef and pol ORFs. In particular. the gp120(UG) sequence shows a 90% homology in the V3 region competed to African HIV-1 strains of the A-clade. This novel approach is extremely effective for the production of VLPs expressing all the epitopes, as confirmed by Western Blot characterization. Furthermore, the resulting HIV-VLP(A)s show the expected density(1.14-1.18 g/ml on a 10-60% sucrose gradient and the morphology of an immature virion at standard transmission electron microscopy. Our results demonstrate that this strategy is highly efficient for expressing a balanced amount of multiple epitopes and their packaging in VLP structures, without affecting the Pr55(gag) autoassembling capacities. Furthermore, the genetic transposition performed in a modified E. coli represents a methodological improvement. allowing a faster and more reproducible identification of recombinant Baculovirus DNA molecules. (C) 2001 Elsevier Science B.V. All rights reserved.
| Item Type: | Article |
|---|---|
| Uncontrolled Keywords: | HUMAN-IMMUNODEFICIENCY-VIRUS; TYPE-1 ENVELOPE GLYCOPROTEIN; CYTOTOXIC T-CELLS; GAG-ENV PARTICLES; NEUTRALIZING ANTIBODIES; MONOCLONAL-ANTIBODIES; INFECTED CELLS; CHALLENGE; PROTECTION; CHIMPANZEES; vaccine, HIV-1; clade-A; VLPs; baculovirus |
| Subjects: | 600 Technology > 610 Medical sciences Medicine |
| Divisions: | Medicine > Lehrstuhl für Medizinische Mikrobiologie und Hygiene |
| Depositing User: | Dr. Gernot Deinzer |
| Date Deposited: | 22 Feb 2022 10:45 |
| Last Modified: | 22 Feb 2022 10:45 |
| URI: | https://pred.uni-regensburg.de/id/eprint/41826 |
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