Grossmann, Johannes and Artinger, Monika and Grasso, Adam W. and Kung, Hsing-Jien and Schoelmerich, Juergen and Fiocchi, Claudio and Levine, Alan D. (2001) Hierarchical cleavage of focal adhesion kinase by caspases alters signal transduction during apoptosis of intestinal epithelial cells. GASTROENTEROLOGY, 120 (1). pp. 79-88. ISSN 0016-5085
Full text not available from this repository.Abstract
Background & Aims: Purified intestinal epithelial cells die of detachment-induced apoptosis due to loss of cell anchorage during isolation, Anchorage-dependent cells form focal adhesions, sites of enhanced cell-matrix attachment that confer survival signals, Focal adhesion kinase (FAK), a component of the focal adhesion signaling complex, transduces these antiapoptotic signals, In this report, the molecular events leading to cleavage of FAK by caspases during apoptosis and its functional implications are defined. Methods: Cytosolic extracts of human intestinal epithelial cells undergoing detachment-induced apoptosis were analyzed by Western blotting immunoprecipitation, and kinase assay, Results: FAK is cleaved by the ordered proteolytic activity of 2 different members of the caspase-3 family, The first cleavage is mediated by caspase-3, generating a 94/92-kilodalton-terminal fragment, which is processed by caspase-6 to an 84-kilodalton fragment. After apoptosis is initiated, the level of FAK phosphorylation is rapidly decreased, and the phosphorylation pattern of FAK-associated proteins is dramatically modified, showing significant yet divergent changes in signal transduction, Conclusions: Cleavage of FAH during apoptosis of normal human cells is an example of the sequential, highly regulated, and coordinate action of caspases that not only dismantle a cell by proteolysis, but also alter the cell's signaling machinery.
| Item Type: | Article |
|---|---|
| Uncontrolled Keywords: | EXTRACELLULAR-MATRIX; MEDIATED ACTIVATION; PROTEIN-KINASE; DEATH; IDENTIFICATION; DISRUPTION; PP125(FAK); MECHANISM; INTEGRINS; PP125FAK; |
| Subjects: | 600 Technology > 610 Medical sciences Medicine |
| Divisions: | Medicine > Lehrstuhl für Innere Medizin I |
| Depositing User: | Dr. Gernot Deinzer |
| Date Deposited: | 28 Feb 2022 10:25 |
| Last Modified: | 28 Feb 2022 10:25 |
| URI: | https://pred.uni-regensburg.de/id/eprint/41858 |
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