Minuth, Will W. and Strehl, Raimund and Schumacher, Karl and de Vries, Uwe (2001) Long term culture of epithelia in a continuous fluid gradient for biomaterial testing and tissue engineering. JOURNAL OF BIOMATERIALS SCIENCE-POLYMER EDITION, 12 (3). pp. 353-365. ISSN 0920-5063
Full text not available from this repository. (Request a copy)Abstract
Epithelia perform barrier functions being exposed to different fluids on the luminal and basal side. For long-term testing of new biomaterials as artificial basement membrane substitutes, it is important to simulate this fluid gradient. Individually-selected biomaterials can be placed in tissue carriers and in gradient containers, where different media are superfused. Epithelia growing on the tissue carriers form a physiological barrier during the whole culture period. Frequently however, pressure differences between the luminal and basal compartments occur. This is caused by a unilateral accumulation of gas bubbles in the container compartments resulting in tissue damage. Consequently, the occurence of gas bubbles has to be minimized. Air bubbles in the perfusion culture medium preferentially acumulate at sites where different materials come into contact. The first development is new screw caps for media bottles, specifically designed to allow fluid contact with only the tube and not the cap material. The second development is the separation of remaining gas bubbles from the liquid phase in the medium using newly-developed gas expander modules. By the application of these new tools, the yield of embryonic renal collecting duct epithelia with intact barrier function on a fragile natural support material can be significantly increased compared to earlier experiments.
| Item Type: | Article |
|---|---|
| Uncontrolled Keywords: | IN-VITRO; CELL-DIFFERENTIATION; PERFUSION CULTURE; RABBIT KIDNEY; CONTAINER; kidney; epithelia; biomaterial testing; tissue engineering; differentiation; perfusion; gradient culture container; electrolytes |
| Subjects: | 500 Science > 570 Life sciences |
| Divisions: | Biology, Preclinical Medicine > Institut für Anatomie > Lehrstuhl für Molekulare und zelluläre Anatomie > Prof. Dr. Will Minuth |
| Depositing User: | Dr. Gernot Deinzer |
| Date Deposited: | 01 Mar 2022 08:10 |
| Last Modified: | 01 Mar 2022 08:10 |
| URI: | https://pred.uni-regensburg.de/id/eprint/41885 |
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