Kellner, K. and Schulz, M. B. and Goepferich, Achim and Blunk, Torsten (2001) Insulin in tissue engineering of cartilage: A potential model system for growth factor application. JOURNAL OF DRUG TARGETING, 9 (6). pp. 439-448. ISSN 1061-186X, 1029-2330
Full text not available from this repository. (Request a copy)Abstract
Investigation of novel experimental application systems for growth factors or other bioactive substances in tissue engineering is often limited by high costs of substances and would benefit from a defined and easily controllable model tissue system. Herein, we demonstrate a potential three-dimensional in vitro system using engineered cartilage as a model tissue and readily available insulin as a model drug. Previously it has been shown that insulin-like growth factor-I (IGF-I) has profound effects on tissue-engineered cartilage in vitro. Insulin is known to bind to the IGF-I receptor and to elicit significant responses in cartilage. In this study, bovine articular chondrocytes were seeded onto biodegradable, polyglycolic acid (PGA) scaffolds and cultured for up to 7 weeks. Exogenous insulin (0.05-50 mug/ml) increased the growth rate and the glycosaminoglycan fraction of tissue-engineered cartilage, decreased the cell number in the tissue constructs, and improved the morphological appearance, with 2.5 mug/ml being the most favorable concentration, The observed effects of insulin were similar to effects of IGF-I (0.05 mug/ml) and were in agreement with the reported binding constants of IGF-I and insulin at the IGF-I receptor. Besides the possibility to employ insulin as a potent substance to improve tissue-engineered cartilage, the presented easily controllable in vitro system may be used in the future to evaluate experimental growth factor application devices using economically favorable insulin as a model protein.
| Item Type: | Article |
|---|---|
| Uncontrolled Keywords: | FACTOR DELIVERY; INVITRO; COLLAGEN; RECEPTOR; INVIVO; cartilage; drug delivery; growth factors; IGF-I; insulin; tissue engineering |
| Subjects: | 600 Technology > 615 Pharmacy |
| Divisions: | Chemistry and Pharmacy > Institute of Pharmacy > Pharmaceutical Technology (Prof. Göpferich) |
| Depositing User: | Dr. Gernot Deinzer |
| Date Deposited: | 01 Mar 2022 09:20 |
| Last Modified: | 01 Mar 2022 09:20 |
| URI: | https://pred.uni-regensburg.de/id/eprint/41889 |
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