Fowler, Susan J. and Jose, Sheba and Zhang, Xiaoming and Deutzmann, Rainer and Sarras, Michael P. and Boot-Handford, Raymond P. (2000) Characterization of hydra type IV collagen - Type IV collagen is essential for head regeneration and its expression is up-regulated upon exposure to glucose. JOURNAL OF BIOLOGICAL CHEMISTRY, 275 (50). pp. 39589-39599. ISSN 0021-9258
Full text not available from this repository. (Request a copy)Abstract
Hydra vulgaris mesoglea is a primitive basement membrane that also exhibits some features of an interstitial matrix. We have characterized cDNAs that encode the full-length hydra alpha1(IV) chain. The 5169-base pair transcript encodes a protein of 1723 amino acids, including an interrupted 1455-residue collagenous domain and a 228-residue C-terminal noncollagenous domain. N-terminal sequence analyses of collagen IV peptides suggest the molecule is homotrimeric. Denatured hydra type IV collagen protein occurs as dimers and higher order aggregates held together by nonreducible cross-links. Hydra collagen TV exhibits no functional evidence for the presence of a 7 S domain. Type TV collagen is expressed by the ectoderm along the entire longitudinal axis of the animal but is most intense at the base of the tentacles at the site of battery cell transdifferentiation. Antisense studies show that inhibition of collagen IV translation causes a blockage in head regeneration, indicating its importance in normal hydra development. Exposure of adult hydra to 15 mm glucose resulted in up-regulation of type IV collagen mRNA levels within 48 h and significant thickening of the mesoglea within 14 days, suggesting that basement membrane thickening seen in diabetes may be, in evolutionary terms, an ancient glucose-mediated response.
| Item Type: | Article |
|---|---|
| Uncontrolled Keywords: | BASEMENT-MEMBRANE COLLAGEN; EXTRACELLULAR-MATRIX MESOGLEA; AMINO-ACID SEQUENCE; STRUCTURAL ORGANIZATION; CAENORHABDITIS-ELEGANS; ALPHA-2(IV) COLLAGEN; CHAIN; GENE; LAMININ; IDENTIFICATION; |
| Subjects: | 500 Science > 570 Life sciences |
| Divisions: | Biology, Preclinical Medicine > Institut für Biochemie, Genetik und Mikrobiologie > Lehrstuhl für Biochemie I > Prof. Dr. Rainer Deutzmann |
| Depositing User: | Dr. Gernot Deinzer |
| Date Deposited: | 08 Mar 2022 14:38 |
| Last Modified: | 08 Mar 2022 14:38 |
| URI: | https://pred.uni-regensburg.de/id/eprint/41976 |
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