Multhoff, Gabriele and Pfister, Karin and Botzler, Claus and Jordan, Andreas and Scholz, Regina and Schmetzer, Helga and Burgstahler, Ralf and Hiddemann, Wolfgang (2000) Adoptive transfer of human natural killer cells in mice with severe combined immunodeficiency inhibits growth of Hsp70-expressing tumors. INTERNATIONAL JOURNAL OF CANCER, 88 (5). pp. 791-797. ISSN 0020-7136
Full text not available from this repository. (Request a copy)Abstract
In vitro, tumor-selective Hsp70 plasma membrane localization correlates with increased sensitivity to lysis mediated by a subpopulation of human natural killer (NK) cells that adhere to plastic following cytokine stimulation. In the present study, we analyzed the capacity of adoptively transferred human NK cells in SCID/beige mice for local tumor control and prevention of metastatic dissemination of Hsp70-expressing CX+ and non-expressing CX- tumors following orthotopic (o.t.) injection. Both tumor sublines were derived by cell sorting of the original cell line, CX2, and thus exhibit an identical MHC and adhesion molecule expression pattern but differ with respect to Hsp70 plasma membrane expression. Viability of adherent, human NK cells in SCID/beige mice up to 18 days and the capacity to migrate have been demonstrated. Growth of Hsp70-expressing and non-expressing CX+ and CX- tumor cells was completely suppressed when 10 x 10(6) NK cells were injected into the i.p. cavity on day 4 after inoculation of 2.5 x 10(6) tumor cells, Although a single injection of 5 or 2.5 x 10(6) NK cells was not sufficient to suppress tumor growth completely in all mice, the reduction in size of CX+ tumors was significantly greater than that of CX- tumors. To mimic the clinical situation, ex vivo stimulated NK cells were injected i.v. on day 4 after o.t. injection of tumor cells. Under these conditions, growth of Hsp70-expressing primary tumors and metastases was suppressed. If CX tumor cells were injected, 3 of 9 mice developed Hsp70-negative primary tumors. However, none of these mice developed distant metastases, In summary, our data indicate that Hsp70 acts as a recognition structure for adherent NK cells in a SCID/beige mouse model. (C) 2000 Wiley-Liss, Inc.
| Item Type: | Article |
|---|---|
| Uncontrolled Keywords: | HEAT-SHOCK-PROTEIN; CYTOTOXIC T-LYMPHOCYTES; HIGH-DOSE INTERLEUKIN-2; STUDY-GROUP TRIAL; SURFACE EXPRESSION; MALIGNANT-MELANOMA; ADVANCED CANCER; NK CELLS; IMMUNOTHERAPY; METASTASIS; |
| Subjects: | 600 Technology > 610 Medical sciences Medicine |
| Divisions: | Medicine > Lehrstuhl für Chirurgie Medicine > Lehrstuhl für Innere Medizin III (Hämatologie und Internistische Onkologie) |
| Depositing User: | Dr. Gernot Deinzer |
| Date Deposited: | 14 Mar 2022 10:54 |
| Last Modified: | 14 Mar 2022 10:54 |
| URI: | https://pred.uni-regensburg.de/id/eprint/42007 |
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