Vogler, Stefanie and Grosche, Antje and Pannicke, Thomas and Wiedemann, Peter and Reichenbach, Andreas and Bringmann, Andreas (2016) Endothelins Inhibit Osmotic Swelling of Rat Retinal Glial and Bipolar Cells by Activation of Growth Factor Signaling. NEUROCHEMICAL RESEARCH, 41 (10). pp. 2598-2606. ISSN 0364-3190, 1573-6903
Full text not available from this repository. (Request a copy)Abstract
Water accumulation in retinal glial (Muller) and neuronal cells resulting in cellular swelling contributes to the development of retinal edema and neurodegeneration. Here, we show that endothelin-1 (ET-1) dose-dependently inhibits the hypoosmotic swelling of Muller cells in freshly isolated retinal slices of control and diabetic rats, with a maximal inhibition at 100 nM. Osmotic Muller cell swelling was also inhibited by ET-2. The effect of ET-1 was mediated by activation of ETA and ETB receptors resulting in transactivation of metabotropic glutamate receptors, purinergic P2Y(1), and adenosine A(1) receptors. ET-1 (but not ET-2) also inhibited the osmotic swelling of bipolar cells in retinal slices, but failed to inhibit the swelling of freshly isolated bipolar cells. The inhibitory effect of ET-1 on the bipolar cell swelling in retinal slices was abrogated by inhibitors of the FGF receptor kinase (PD173074) and of TGF-beta 1 superfamily activin receptor-like kinase receptors (SB431542), respectively. Both Muller and bipolar cells displayed immunoreactivities of ETA and ETB receptor proteins. The data may suggest that neuroprotective effects of ETs in the retina are in part mediated by prevention of the cytotoxic swelling of retinal glial and bipolar cells. ET-1 acts directly on Muller cells, while the inhibitory effect of ET-1 on bipolar cell swelling is indirectly mediated, via stimulation of the release of growth factors like bFGF and TGF-beta 1 from Muller cells.
| Item Type: | Article |
|---|---|
| Uncontrolled Keywords: | OPTIC-NERVE; IN-VIVO; GANGLION-CELLS; DIABETIC-RATS; B RECEPTORS; MULLER; RELEASE; GLAUCOMA; VOLUME; INJURY; Endothelin; Cell swelling; Muller cell; Bipolar cell; Diabetic retinopathy; Retina |
| Subjects: | 600 Technology > 610 Medical sciences Medicine |
| Divisions: | Medicine > Lehrstuhl für Humangenetik |
| Depositing User: | Dr. Gernot Deinzer |
| Date Deposited: | 05 Apr 2019 11:38 |
| Last Modified: | 05 Apr 2019 11:38 |
| URI: | https://pred.uni-regensburg.de/id/eprint/4202 |
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