Castrop, Hayo and Kammerl, Martin and Mann, Bianca and Jensen, Boye L. and Kraemer, Bernhard K. and Kurtz, Armin (2000) Cyclooxygenase 2 and neuronal nitric oxide synthase expression in the renal cortex are not interdependent in states of salt deficiency. PFLUGERS ARCHIV-EUROPEAN JOURNAL OF PHYSIOLOGY, 441 (2-3). pp. 235-240. ISSN 0031-6768
Full text not available from this repository. (Request a copy)Abstract
Neuronal nitric oxide synthase (nNOS) and cyclooxygenase-2 (COX-2) expression in the kidney are localized to the cortical thick ascending limb of the loop of Henle (cTALH), including the macula region, and increase after salt restriction. Because of the similar localization and regulation of nNOS and COX-2 expression, we have examined whether there is a functional interrelationship between the expression of the two enzymes. Male Sprague Dawley rats were fed for 1 week either a low-salt diet (0.02% w/w) which produced model-ate increases of nNOS and COX-2 expression, or low salt combined with the angiotensin I converting enzyme inhibitor ramipril (10 mg/kg per day), which produced strong increases of renocortical nNOS and COX-2 expressions. To inhibit nNOS or COX-2 activities, animals received in addition N-G-nitro-L-arginine methyl ester (L-NAME, 50 mg/kg pel day) or rofecoxib (10 mg/kg per day) for 1 week, respectively. L-NAME treatment did not change COX-2 expression and conversely rofecoxib treatment did not change nNOS expression in the kidney cortex under any experimental conditions. L-NAME but not rofecoxib attenuated renin mRNA levels. Rofecoxib markedly reduced renal prostanoid excretion. These findings suggest that under these conditions the control of nNOS and COX-2 gene expression in the macula densa regions of the kidney cortex are not dependent on each other.
| Item Type: | Article |
|---|---|
| Uncontrolled Keywords: | RENIN GENE-EXPRESSION; MACULA DENSA; RAT-KIDNEY; RENOVASCULAR HYPERTENSION; ANGIOTENSIN-II; INHIBITION; PRESSURE; BIOSYNTHESIS; STIMULATION; SECRETION; prostaglandins; renin |
| Subjects: | 500 Science > 570 Life sciences 600 Technology > 610 Medical sciences Medicine |
| Divisions: | Medicine > Lehrstuhl für Innere Medizin II Biology, Preclinical Medicine > Institut für Physiologie > Prof. Dr. Armin Kurtz Biology, Preclinical Medicine > Institut für Physiologie > Prof. Dr. Wolf Hayo Castrop |
| Depositing User: | Dr. Gernot Deinzer |
| Date Deposited: | 15 Mar 2022 07:13 |
| Last Modified: | 15 Mar 2022 07:13 |
| URI: | https://pred.uni-regensburg.de/id/eprint/42028 |
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