Langmann, Thomas and Porsch-Özcürümez, Mustafa and Unkelbach, Uwe and Klucken, Jochen and Schmitz, Gerd (2000) Genomic organization and characterization of the promoter of the human ATP-binding cassette transporter-G1 (ABCG1) gene. BIOCHIMICA ET BIOPHYSICA ACTA-GENE STRUCTURE AND EXPRESSION, 1494 (1-2). pp. 175-180. ISSN 0167-4781
Full text not available from this repository. (Request a copy)Abstract
The ATP-binding cassette transporter G1 (ABCG1) was recently identified as a regulator of macrophage cholesterol and phospholipid transport. This transporter together with ABCA1 belongs to a group of sterol-sensitive ABC proteins which are induced by lipid loading or specific oxysterols. We report here the genomic structure of ABCG1 along with the 5' flanking sequence using library screening and BLAST search analysis. The ABCG1 gene spans more than 70 kb and contains 15 exons. The exon size is between 30 and 1081 bp and the introns range in size from 137 bp to more than 45 kb. All exon-intron boundaries display the canonical GT/AG sequences. Using promoter-luciferase reporter assays in the myeloid cell lines THP-1 and RAW246.7 and the hepatoma cell line HepG2 we could demonstrate the functionality of the ABCG1 promoter and the minimal sequence requirements for gene expression. The TATA-less proximal promoter contains multiple Sp1 binding sites and a consensus sequence for sterol regulatory element binding protein. (C) 2000 Elsevier Science B.V. All rights reserved.
| Item Type: | Article |
|---|---|
| Uncontrolled Keywords: | DROSOPHILA WHITE GENE; TANGIER-DISEASE; CHROMOSOME 21Q22.3; MOLECULAR-CLONING; MAMMALIAN-CELLS; HUMAN HOMOLOG; CHOLESTEROL; EXPRESSION; MUTATIONS; REGION; ATP-binding cassette transporter G1; genomic organization; promoter region; cholesterol transport; Sp1; sterol regulatory element binding protein |
| Subjects: | 600 Technology > 610 Medical sciences Medicine |
| Divisions: | Medicine > Lehrstuhl für Klinische Chemie und Laboratoriumsmedizin |
| Depositing User: | Dr. Gernot Deinzer |
| Date Deposited: | 15 Mar 2022 10:37 |
| Last Modified: | 15 Mar 2022 10:37 |
| URI: | https://pred.uni-regensburg.de/id/eprint/42056 |
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