Enderlein, Joerg (2000) Tracking of fluorescent molecules diffusing within membranes. APPLIED PHYSICS B-LASERS AND OPTICS, 71 (5). pp. 773-777. ISSN 0946-2171
Full text not available from this repository. (Request a copy)Abstract
A new method is proposed for tracking fluorescing single molecules diffusing within a two-dimensional membrane. It is based on a confocal microscopy setup with a constantly rotating laser focus, which follows the position of the molecule. The optimization and efficiency of the method are theoretically studied for a broad range of experimentally realistic conditions. The proposed method allows for a long-time observation of diffusing molecules while allowing the application of fast spectroscopic techniques such as fluorescence decay time determination or fluorescence anisotropy measurements.
| Item Type: | Article |
|---|---|
| Uncontrolled Keywords: | LASER-INDUCED FLUORESCENCE; SINGLE MOLECULES; NEUTRAL PARTICLES; IDENTIFICATION; |
| Subjects: | 500 Science > 540 Chemistry & allied sciences |
| Divisions: | Chemistry and Pharmacy > Institut für Analytische Chemie, Chemo- und Biosensorik |
| Depositing User: | Dr. Gernot Deinzer |
| Date Deposited: | 15 Mar 2022 14:13 |
| Last Modified: | 15 Mar 2022 14:13 |
| URI: | https://pred.uni-regensburg.de/id/eprint/42069 |
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