Kappes, A. and Loeffler, Georg (2000) Influences of ionomycin, dibutyryl-cycloAMP and tumour necrosis factor-alpha on intracellular amount and secretion of apM1 in differentiating primary human preadipocytes. HORMONE AND METABOLIC RESEARCH, 32 (11-12). pp. 548-554. ISSN 0018-5043, 1439-4286
Full text not available from this repository. (Request a copy)Abstract
3T3-L1-adipocytes produce the adipocyte complement related protein of 30 kD (Acrp30), which is also designated as AdipoQ, In order to study the expression and secretion of the human homologue of this protein, apM1 (adipose Most abundant gene transcript I, also named gelatin-binding protein of 28 kD [GBP28] or adiponectin), a polyclonal antibody was produced. Both expression and secretion can be detected beginning with day 4 after induction of differentiation. The amount of expressed apM1 correlates with the specific activity of the differentiation marker glycerol-3-phosphate dehydrogenase. Secretion of apM1 is increased by the addition of ionomycin. Both the nonhydrolysable dibutyryl-cycloAMP and tumour necrosis factor alpha reduce the expression and secretion of apM1.
| Item Type: | Article |
|---|---|
| Uncontrolled Keywords: | ADIPOSE-SPECIFIC PROTEIN; 3T3-L1 ADIPOCYTES; GLYCEROPHOSPHATE DEHYDROGENASE; INSULIN-RESISTANCE; GENE-EXPRESSION; OBESE GENE; ADIPONECTIN; CLONING; TISSUE; BINDING; ionomycin; dibutyryl-cycloAMP; Tumour Necrosis Factor alpha (TNFalpha) apM1-antibody; Acrp30/AdipoQ; GBP28; adiponectin |
| Subjects: | 500 Science > 570 Life sciences |
| Divisions: | Biology, Preclinical Medicine > Institut für Biochemie, Genetik und Mikrobiologie > Lehrstuhl für Biochemie III |
| Depositing User: | Dr. Gernot Deinzer |
| Date Deposited: | 21 Mar 2022 06:39 |
| Last Modified: | 21 Mar 2022 06:39 |
| URI: | https://pred.uni-regensburg.de/id/eprint/42089 |
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