Goetz, Marc and Roitsch, Thomas (2000) Identification of amino acids essential for enzymatic activity of plant invertases. JOURNAL OF PLANT PHYSIOLOGY, 157 (5). pp. 581-585. ISSN 0176-1617
Full text not available from this repository. (Request a copy)Abstract
The role of two conserved sequence motifs in plant invertases, the NDPN and WEC-P/V-D boxes, was studied by a structure-function analysis of extracellular invertase CIN1 from Chenopodium rubrum. Specific amino acid substitutions were introduced by site-directed mutagenesis, and the mutated genes were heterologously expressed in an invertase deficient Saccharomyces cerevisiae strain. It was shown that the aspartate from the first and the glutamate and the cysteine from the second box are essential for enzyme activity. The possible function of these amino acid residues is discussed with respect to the inhibitory effect of sulfhydryl group blocking reagents on invertase activity, the interaction between amino acids in the active centre, and their participation in the catalytic mechanism.
| Item Type: | Article |
|---|---|
| Uncontrolled Keywords: | CHENOPODIUM-RUBRUM; YEAST INVERTASE; EXPRESSION; PURIFICATION; SUGAR; TRANSPORTER; INDUCTION; GLUCOSE; PROTEIN; LEAVES; invertase isoenzymes; pH-optimum; substrate specificity; site directed mutagenesis |
| Subjects: | 500 Science > 580 Botanical sciences |
| Divisions: | Biology, Preclinical Medicine > Institut für Pflanzenwissenschaften > Lehrstuhl für Zellbiologie und Pflanzenphysiologie (Prof. Dr. Klaus Grasser) |
| Depositing User: | Dr. Gernot Deinzer |
| Date Deposited: | 21 Mar 2022 15:42 |
| Last Modified: | 21 Mar 2022 15:42 |
| URI: | https://pred.uni-regensburg.de/id/eprint/42102 |
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