Graf, Marcus and Bojak, Alexandra and Deml, Ludwig and Bieler, Kurt and Wolf, Hans and Wagner, Ralf (2000) Concerted action of multiple cis-acting sequences is required for Rev dependence of late human immunodeficiency virus type 1 gene expression. JOURNAL OF VIROLOGY, 74 (22). pp. 10822-10826. ISSN 0022-538X
Full text not available from this repository. (Request a copy)Abstract
Based on the human immunodeficiency virus type 1 (HIV-1) gag gene, subgenomic reporter constructs have been established allowing the contributions of different cis-acting elements to the Rev dependency of late HIV-1 gene products to be determined. Modification of intragenic regulatory elements achieved by adapting the codon usage of the complete gene to highly expressed mammalian genes resulted in constitutive nuclear export allowing high levels of Gag expression independent from the Rev/Rev-responsive element system and irrespective of the absence or presence of the isolated major splice donor. Leptomycin B inhibitor studies revealed that the RNAs derived from the codon-optimized gag gene lacking AU-rich inhibitory elements are directed to a distinct, CRM1-independent, nuclear export pathway.
| Item Type: | Article |
|---|---|
| Uncontrolled Keywords: | MEDIATED NUCLEAR EXPORT; ENV MESSENGER-RNA; SPLICE SITES; HIV-1 REV; ELEMENTS; INHIBITION; SIGNAL; INTRON; CRM1; GAG; |
| Subjects: | 600 Technology > 610 Medical sciences Medicine |
| Divisions: | Medicine > Lehrstuhl für Medizinische Mikrobiologie und Hygiene |
| Depositing User: | Dr. Gernot Deinzer |
| Date Deposited: | 21 Mar 2022 15:43 |
| Last Modified: | 21 Mar 2022 15:43 |
| URI: | https://pred.uni-regensburg.de/id/eprint/42106 |
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