Suessmuth, Sigurd D. and Muscholl-Silberhorn, Albrecht and Wirth, Reinhard and Susa, Milorad and Marre, Reinhard and Rozdzinski, Eva (2000) Aggregation substance promotes adherence, phagocytosis, and intracellular survival of Enterococcus faecalis within human macrophages and suppresses respiratory burst. INFECTION AND IMMUNITY, 68 (9). pp. 4900-4906. ISSN 0019-9567
Full text not available from this repository. (Request a copy)Abstract
The aggregation substance (AS) of Enterococcus faecalis, encoded on sex pheromone plasmids, is a surface-bound glycoprotein that mediates aggregation between bacteria thereby facilitating plasmid transfer. Sequencing of the pAD1-encoded Asa1 revealed that this surface protein contains two RGD motifs which are known to ligate integrins. Therefore, we investigated the influence of AS on the interaction of E. faecalis with human monocyte-derived macrophages which constitutively express beta(2) integrins (e.g., CD18). AS was found to cause a greater-than-fivefold increase in enterococcal adherence to macrophages and a greater-than-sevenfold increase in phagocytosis. Adherence was mediated by an interaction between the RGD motif and the integrin CD11b/CD18 (complement receptor type 3) as demonstrated by inhibition studies with monoclonal antibodies and RGD peptide. AS-bearing enterococci were significantly more resistant to macrophage killing during the first 3 h postinfection, probably due to inhibition of the respiratory burst as indicated by reduced concentrations of superoxide anion.
| Item Type: | Article |
|---|---|
| Uncontrolled Keywords: | SEX-PHEROMONE; STREPTOCOCCUS-FAECALIS; POLYMORPHONUCLEAR LEUKOCYTES; PERITONEAL-MACROPHAGES; HUMAN NEUTROPHILS; PLASMID TRANSFER; HUMAN-MONOCYTES; CELL-ADHESION; RECEPTOR; INTEGRINS; |
| Subjects: | 500 Science > 570 Life sciences |
| Divisions: | Biology, Preclinical Medicine > Institut für Biochemie, Genetik und Mikrobiologie > Lehrstuhl für Mikrobiologie (Archaeenzentrum) > Prof. Dr. Reinhard Wirth |
| Depositing User: | Dr. Gernot Deinzer |
| Date Deposited: | 28 Mar 2022 06:50 |
| Last Modified: | 28 Mar 2022 06:50 |
| URI: | https://pred.uni-regensburg.de/id/eprint/42235 |
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