Castrop, Hayo and Kraemer, Bernhard K. and Riegger, Guenter A. J. and Kurtz, Armin and Wolf, Konrad (2000) Overexpression of chloride channel CLC-K2 mRNA in the renal medulla of Dahl salt-sensitive rats. JOURNAL OF HYPERTENSION, 18 (9). pp. 1289-1295. ISSN 0263-6352
Full text not available from this repository. (Request a copy)Abstract
Objective The present study aimed to characterize the influence of salt intake on the gene expression of the kidney specific chloride channels CLC-K1 and CLC-K2 in the kidneys of salt-resistant and salt-sensitive Dahl rats. Design For this purpose Dahl salt-resistant (Dahl-R) and Dahl salt-sensitive rats (Dahl-S) were fed a low (0.02%), normal (0.6%) or high (4%) salt diet for 19 days and CLC-K1 and -K2 mRNA expression was semiquantitated in cortex, outer and inner medulla, Methods Kidneys were macroscopically dissected, total RNA was isolated according to the guanidinium-thiocyanate-phenol-chloroform method and messenger RNAs for the kidney specific chloride channels CLC-K1 and CLC-K2 were measured by ribonuclease protection assay. Results Systolic blood pressure in high salt-treated Dahl-S rats increased to 204 +/- 5 mmHg Versus 150 +/- 7 mmHg in Dahl-S controls. Dahl R and low salt Dahl-S rats showed no increase in blood pressure. For CLC-K1 mRNA we found an order of abundance inner medulla >> outer medulla >> cortex. There was no difference in mRNA abundance between Dahl-R and -S, nor any effect of the rate of salt intake on CLC-K1 mRNA abundance in the different kidney zones. CLC-K2 mRNA expression in cortex and outer medulla was similar between Dahl-R and -S rats. In the inner medulla, however, CLC-K2 mRNA was 1.7-fofd higher in Dahl-S than in Dahl-R rats. In the cortex we found no influence of salt intake on CLC-K2 mRNA. In outer and inner medulla of Dahl-R rats and Dahl-S rats high salt diet led to a marked downregulation of CLC-K2 mRNA expression. Consequently, CLC-K2 gene expression in the inner medulla was 2.2-fold higher in Dahl-S than in Dahl-R rats in states of high salt diet Conclusion Given that the CLC-K2 chloride channel in the outer and inner medulla contributes to salt reabsorption, our findings would suggest that Dahl-S rats have an increased medullary salt reabsorption. This may contribute to the inability of these animals to excrete an increased salt load at a normal renal perfusion pressure leading to the development of hypertension. J Hypertens 2000, 18:1289-1295 (C) Lippincott Williams & Wilkins.
| Item Type: | Article |
|---|---|
| Uncontrolled Keywords: | KIDNEY; HYPERTENSION; TRANSPLANTATION; GENE; hypertension; kidney; sodium chloride channel |
| Subjects: | 500 Science > 570 Life sciences 600 Technology > 610 Medical sciences Medicine |
| Divisions: | Medicine > Lehrstuhl für Innere Medizin II Biology, Preclinical Medicine > Institut für Physiologie > Prof. Dr. Armin Kurtz |
| Depositing User: | Dr. Gernot Deinzer |
| Date Deposited: | 28 Mar 2022 08:10 |
| Last Modified: | 28 Mar 2022 08:10 |
| URI: | https://pred.uni-regensburg.de/id/eprint/42241 |
Actions (login required)
 |
View Item |
