Fritsche, Jana and Moser, Markus and Faust, Stefan and Peuker, Alice and Buettner, Reinhard and Andreesen, Reinhard and Kreutz, Marina (2000) Molecular cloning and characterization of a human metalloprotease disintegrin - a novel marker for dendritic cell differentiation. BLOOD, 96 (2). pp. 732-739. ISSN 0006-4971, 1528-0020
Full text not available from this repository. (Request a copy)Abstract
The 1 alpha,25-dihydroxyvitamin D-3 (1,25-[OH](2)VD3) modulates the differentiation of monocytic cell lines and monocytes (MOs) in vitro. Up to now several target genes of 1,25(OH)(2)VD3 have been described in monocytic cell lines; however, little is known about target genes in primary MOs. With the Differential Display technique, we found a transcript upregulated by 1,25(OH)(2)VD3 in short-term cultured human blood MOs, which we called MADDAM (metalloprotease and disintegrin dendritic antigen marker; EMBL/GenBank/DDBJ accession no. Y13786), Northern blot analysis confirmed this result and revealed a signal of MADDAM messenger RNA (mRNA) at about 7.5 kilobases (kb), Long-term culture (more than 20 hours) of MOs during macrophage (MAC) differentiation led to a rapid and complete down-regulation of MADDAM expression. In contrast, MADDAM expression was maintained in MOs differentiated along the dendritic cell (DC) pathway and Induced in CD34(+)-derived Dos, In addition, in situ hybridization revealed signals of MADDAM mRNA in follicles of human lymph nodes and MADDAM mRNA was detected In freshly isolated human blood-DCs by reverse transcription-polymerage chain reaction (RT-PCR), By means of a database search, we found that MADDAM is a member of the ADAM (a metalloprotease and disintegrin) family, the human homologue to murine meltrin-beta (ADAM 19), From these data, we conclude that MADDAM is an important marker for the differentiation and characterization of DCs and the distinction between MACs end Dos.
| Item Type: | Article |
|---|---|
| Uncontrolled Keywords: | COLONY-STIMULATING FACTOR; NECROSIS-FACTOR-ALPHA; PERIPHERAL-BLOOD LYMPHOCYTES; POLYMERASE CHAIN-REACTION; HUMAN-MONOCYTES; IN-VITRO; MACROPHAGE MATURATION; ACCESSORY CELLS; ADAM FAMILY; TNF-ALPHA; |
| Subjects: | 600 Technology > 610 Medical sciences Medicine |
| Divisions: | Medicine > Abteilung für Pädiatrische Hämatologie, Onkologie und Stammzelltransplantation Medicine > Lehrstuhl für Pathologie |
| Depositing User: | Dr. Gernot Deinzer |
| Date Deposited: | 30 Mar 2022 12:44 |
| Last Modified: | 30 Mar 2022 12:44 |
| URI: | https://pred.uni-regensburg.de/id/eprint/42321 |
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