Hauptmann, Hagen and Paulus, Birgit and Kaiser, Thomas and Luppa, Peter B. (2000) Concepts for the syntheses of biotinylated steroids. Part II: 17 beta-estradiol derivatives as immunochemical probes. BIOCONJUGATE CHEMISTRY, 11 (4). pp. 537-548. ISSN 1043-1802
Full text not available from this repository. (Request a copy)Abstract
Biotinylated 17 beta-estradiol (E2) derivatives are helpful probes for a better understanding of biospecific E2 interactions with steroid-binding proteins such as the estrogen receptor and anti-steroid antibodies. We describe synthetic strategies for the biotinylation of E2 toward the 3, 6 alpha, and 7 alpha positions using biotinyl-N-hydroxysuccinimide esters with different spacers, varying in structure and chain length. Key reaction for biotinylation at the 3 position is the regioselective ether formation of the phenolate E2 anion with a linker mesylate without protecting the 17 beta-hydroxyl group. The 6 alpha position is accessible via a 3,17 beta protected 6 alpha-hydroxy E2, prepared by stereospecific sodium borohydride reduction of 6-oxo E2. Direct cyanoethylation of the alcohol followed by reduction to the amine allows the biotinylation to 6 alpha-O-coupled cyanoethyloxy linker E2 derivatives. Alternatively, 6 alpha-O-coupled cyanoalkyloxy polyether linker E2 probes are obtained by a Williamson ether synthesis of the alcohol precursor with omega-t-butyl-dimethylsilyloxy-5-oxa-nonylmesylate. Cyanoethylation of the desilylated compound and further reduction of the nitrile led to the terminal amine. Reductive amination of the 3,17 beta acetylated 6-oxo E2 compound with 6-cyanoethyloxyhexyl ammonium acetate yields in a mixture of 6 alpha/beta-N-alkylated E2 nitriles. The epimers are separated by reversed-phase HPLC and the 6 alpha-compound subsequently reduced to the terminal amine. The 7 alpha-biotinylated E2 compound is derived from 7 alpha-(11'-undecyl-N-methyl-N-butylamide) E2, which is already known from literature. Subsequently, the 3 and 17 beta positions are protected, and the amide is reduced to the 7 alpha-(11'-undecanol) compound. Further cyanoethylation and reduction led to the 11'-amino-ethyloxyundecyl E2. Using H-1 NMR analysis, it could be shown that the biotin moiety of the biotinylated 6 alpha- and 7 alpha-E2 derivatives has an axial position which results in a vertical orientation of the substituent toward the cl-face of the planar tetracyclic backbone. Thus, a negligible alteration of the original structure of the upper beta-face offers the feasibility of applying the 6 alpha- and 7 alpha-derivatives as optimal tracers in competitive immunoassays.
| Item Type: | Article |
|---|---|
| Uncontrolled Keywords: | COMPETITIVE CHEMILUMINESCENCE IMMUNOASSAY; CHEMI-LUMINESCENCE IMMUNOASSAY; ESTRADIOL DERIVATIVES; ESTROGEN-RECEPTOR; TESTOSTERONE DERIVATIVES; MONOCLONAL-ANTIBODIES; MOLECULAR-BASIS; SPECIFICITY; BINDING; PROGESTERONE; |
| Subjects: | 500 Science > 540 Chemistry & allied sciences |
| Divisions: | Chemistry and Pharmacy > Institut für Organische Chemie |
| Depositing User: | Dr. Gernot Deinzer |
| Date Deposited: | 05 Apr 2022 05:51 |
| Last Modified: | 05 Apr 2022 05:51 |
| URI: | https://pred.uni-regensburg.de/id/eprint/42335 |
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