Muscholl-Silberhorn, Albrecht B. (2000) Pheromone-regulated expression of sex pheromone plasmid pAD1-encoded aggregation substance depends on at least six upstream genes and a cis-acting, orientation-dependent factor. JOURNAL OF BACTERIOLOGY, 182 (13). pp. 3816-3825. ISSN 0021-9193
Full text not available from this repository. (Request a copy)Abstract
Conjugative transfer of Enterococcus faecalis-specific sex pheromone plasmids relies on an adhesin, called aggregation substance, to confer a tight cell-to-cell contact between the mating partners. To analyze the dependence of pAD1-encoded aggregation substance, Asa1, on pheromone induction, a variety of upstream fragments were fused to an alpha-amylase reporter gene, amyL, by use of a novel promoter probe vector, pAMY-em1, For pheromone-regulated alpha-amylase activity, a total of at least six genes, traB, traC, traA, traE1, orfY, and orf1, are required: TraB efficiently represses asa1 (by a mechanism unrelated to its presumptive function in pheromone shutdown, since a complete shutdown is observed exclusively in the presence of traC); only traC can relieve traB-mediated repression in a pheromone-dependent manner. In addition to traB, traA is required but not sufficient for negative control. Mutational inactivation of traE1, orfY, or orf1, respectively, results in a total loss of or-amylase activity for constructs normally mediating constitutive expression, Inversion of a fragment covering traA, P-0, and traE1 without disrupting any gene or control element switches off amyL or asa1 expression, indicating the involvement of a cis-acting, orientation dependent factor las had been shown for plasmid pCF10), Unexpectedly, pAD1 represses all pAMY-em1 derivatives in trans, while its own pheromone-dependent functions are unaffected. The discrepancy between the new data and those of former studies defining TraE1 as a trans-acting positive regulator is discussed.
| Item Type: | Article |
|---|---|
| Uncontrolled Keywords: | ENTEROCOCCUS-FAECALIS; STREPTOCOCCUS-FAECALIS; CONJUGATION FUNCTIONS; SUBINHIBITORY CONCENTRATIONS; INDUCIBLE CONJUGATION; NUCLEOTIDE-SEQUENCE; PAD1; PROTEIN; REGION; CELLS; |
| Subjects: | 500 Science > 570 Life sciences |
| Divisions: | Biology, Preclinical Medicine > Institut für Biochemie, Genetik und Mikrobiologie > Lehrstuhl für Mikrobiologie (Archaeenzentrum) |
| Depositing User: | Dr. Gernot Deinzer |
| Date Deposited: | 05 Apr 2022 08:29 |
| Last Modified: | 05 Apr 2022 08:29 |
| URI: | https://pred.uni-regensburg.de/id/eprint/42352 |
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