Hofmann, Markus and Roitsch, Thomas (2000) The hexokinase inhibitor glucosamine exerts a concentration dependent dual effect on protein kinase activity in vitro. JOURNAL OF PLANT PHYSIOLOGY, 157 (1). pp. 13-16. ISSN 0176-1617
Full text not available from this repository. (Request a copy)Abstract
Hexokinase inhibitors are used as tools to address the function of hexokinase in sugar dependent signal transduction pathways of plants. We have characterised glucosamine, N-acetyl-glucosamine and manno-heptulose for their ability to inhibit hexokinase and found pronounced differences in the inhibition of glucose-phosphorylating activity. As it is known that phosphorylation reactions are involved in sugar signaling, we have also evaluated the effect of the three hexokinase inhibitors on the activity of a glucose activated MAP kinase purified from tomato suspension culture cells. Glucosamine was shown not only to be a weak hexokinase inhibitor but specifically affected protein kinase activity. Depending on the glucosamine concentration, the in vitro activity of the protein kinase was stimulated or inhibited. In contrast, the hexokinase inhibitors mannoheptulose and N-acetylglucosamine did not show this effect.
| Item Type: | Article |
|---|---|
| Uncontrolled Keywords: | ALPHA-AMYLASE GENE; HIGHER-PLANTS; SUGAR SENSOR; EXPRESSION; CELLS; PHOSPHORYLATION; TRANSDUCTION; REPRESSION; EMBRYOS; GROWTH; Lycopersicon peruvianum; glucosamine; hexokinase inhibitor; MAP kinase |
| Subjects: | 500 Science > 580 Botanical sciences |
| Divisions: | Biology, Preclinical Medicine > Institut für Pflanzenwissenschaften > Lehrstuhl für Zellbiologie und Pflanzenphysiologie (Prof. Dr. Klaus Grasser) |
| Depositing User: | Dr. Gernot Deinzer |
| Date Deposited: | 05 Apr 2022 09:59 |
| Last Modified: | 05 Apr 2022 09:59 |
| URI: | https://pred.uni-regensburg.de/id/eprint/42360 |
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