Inoue, Kyoko and Yamada, Hiroaki and Akasaka, Kazuyuki and Herrmann, Christian and Kremer, Werner and Maurer, Till and Döker, Rolf and Kalbitzer, Hans Robert (2000) Pressure-induced local unfolding of the Ras binding domain of RalGDS. NATURE STRUCTURAL BIOLOGY, 7 (7). pp. 547-550. ISSN 1072-8368
Full text not available from this repository.Abstract
The reliable prediction of the precise three-dimensional structure of proteins from their amino acid sequence is a major, still unresolved problem in biochemistry. Pressure is a parameter that controls folding/unfolding transitions of proteins through the volume change Delta V of the protein-solvent system. By varying the pressure from 30 to 2,000 bar we detected using N-15/H-1 2D NMR spectroscopy a unique equilibrium unfolding intermediate I in the Ras binding domain of the Ral guanine nucleotide dissociation stimulator (Ral GDS). It is characterized by a local melting of specific structural elements near hydrophobic cavities while the overall folded structure is maintained.
| Item Type: | Article |
|---|---|
| Uncontrolled Keywords: | CHEMICAL-SHIFTS; HIGH-RESOLUTION; NMR; PROTEINS; H-1; DENATURATION; SENSITIVITY; DEPENDENCE; LYSOZYME; DYNAMICS |
| Subjects: | 500 Science > 570 Life sciences |
| Divisions: | Biology, Preclinical Medicine > Institut für Biophysik und physikalische Biochemie > Prof. Dr. Dr. Hans Robert Kalbitzer |
| Depositing User: | Dr. Gernot Deinzer |
| Date Deposited: | 24 Aug 2023 09:31 |
| Last Modified: | 24 Aug 2023 09:31 |
| URI: | https://pred.uni-regensburg.de/id/eprint/42366 |
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