Sibayan, Santiago A. B. and Kobuch, Karin and Spiegel, Detlev and Eckert, Elfriede and Leser, Rita and Monzer, J. and Gabel, Veit-Peter (2000) Epinephrine, but not dexamethasone, induces apoptosis in retinal pigment epithelium cells in vitro: possible implications an the pathogenesis of central serous chorioretinopathy. GRAEFES ARCHIVE FOR CLINICAL AND EXPERIMENTAL OPHTHALMOLOGY, 238 (6). pp. 515-519. ISSN 0721-832X
Full text not available from this repository.Abstract
Background: The pathogenesis of central serous chorioretinopathy is poorly understood. It is believed to be due to dysfunction of the retinal pigment epithelium and/or choroid and has been associated with elevated levels of epinephrine and administration of corticosteroids. Epinephrine and corticosteroids have previously been shown to induce apoptosis (programmed cell death) in various types of cells. The objective of this study was to investigate whether these agents can induce apoptosis in cultured retinal pigment epithelium cells. This may help elucidate the pathogenesis of central serous chorioretinopathy. Methods: Third-passage porcine retinal pigment epithelium cells were grown to confluence and incubated for 1-7 days in culture medium containing epinephrine (10(2)-10(9) pg/ml) or a corticosteroid, dexamethasone (4-4x10(4) ng/ml). The cultures were evaluated for apoptosis by phase-contrast microscopy and in situ terminal deoxynucleotidyl transferase-mediated dUTP nick-end labeling. Results: Epinephrine (7x10(7)-10(9) pg/ml) induced apoptosis in a dose- and time-dependent manner. Exposure to lower concentrations of epinephrine(10(2)-6x10(7) pg/ml) and all tested levels of dexamethasone did not result in apoptosis. Conclusion: Retinal pigment epithelium cells may undergo apoptosis following exposure to elevated levels of epinephrine. These findings suggest a possible pathophysiologic mechanism for the development of central serous chorioretinopathy.
| Item Type: | Article |
|---|---|
| Uncontrolled Keywords: | SYSTEMIC CORTICOSTEROID TREATMENT; DNA FRAGMENTATION; GROWTH-FACTOR; DEATH; GLUCOCORTICOIDS; ENDONUCLEASE; RETINOPATHY; ACTIVATION; INVIVO; |
| Subjects: | 600 Technology > 610 Medical sciences Medicine |
| Divisions: | Medicine > Lehrstuhl für Augenheilkunde |
| Depositing User: | Dr. Gernot Deinzer |
| Date Deposited: | 26 Apr 2022 10:52 |
| Last Modified: | 26 Apr 2022 10:52 |
| URI: | https://pred.uni-regensburg.de/id/eprint/42426 |
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