Kloth, Sabine and Kobuch, K. and Domokos, J. and Wanke, C. and Monzer, J. (2000) Polar application of test substances in organotypic environment and under continuous medium flow: A new tissue-based test concept for a broad range of applications in pharmacotoxicology. TOXICOLOGY IN VITRO, 14 (3). pp. 265-274. ISSN 0887-2333
Full text not available from this repository. (Request a copy)Abstract
We have established a new test concept for in vitro pharmacological trials. Our model employs tissue explants to test compounds for toxicity which arises with the metabolic interactions among different cell types. Microsurgical preparation of tissue explants avoids the destruction of the organ-specific tissue architecture. Explants were mounted in tissue carriers to improve nutrition and handling of the sample. To allow for the omission of serum supplementation of the culture medium, explants were cultured under continuous medium flow. Test substances are applied considering the polar architecture of most tissues in vivo, for example, covering the apical aspect of epithelia, In principal, all tissues obtained from any species, including man, can be used in this system. A trial application was performed with vitreous body substitutes, substances used in ophthalmology. One compound had passed cell culture tests, but caused massive blood vessel deterioration in vivo. Using our test system based on the developing renal vessel system, we could confirm, within 24 hours, severe vessel damage which resembles the injury suffered by the rabbit retina. We demonstrate that an improved tissue culture assay is a suitable tool for the detection of toxicity that remained unidentified in cell culture tests. (C) 2000 Elsevier Science Ltd. All rights reserved.
| Item Type: | Article |
|---|---|
| Uncontrolled Keywords: | PERFUSION CULTURE; IN-VITRO; TOXICITY; CELLS; CYTOTOXICITY; RECOMMENDATIONS; INVITRO; ASSAYS; KIDNEY; toxicology; biocompatibility; in vitro test model; serum-free perfusion culture |
| Subjects: | 500 Science > 570 Life sciences 600 Technology > 610 Medical sciences Medicine |
| Divisions: | Medicine > Lehrstuhl für Augenheilkunde Biology, Preclinical Medicine > Institut für Anatomie |
| Depositing User: | Dr. Gernot Deinzer |
| Date Deposited: | 03 May 2022 08:41 |
| Last Modified: | 03 May 2022 08:41 |
| URI: | https://pred.uni-regensburg.de/id/eprint/42473 |
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