Oswald, Bernhard and Lehmann, Frank and Simon, Lydia and Terpetschnig, Ewald and Wolfbeis, Otto S. (2000) Red laser-induced fluorescence energy transfer in an immunosystem. ANALYTICAL BIOCHEMISTRY, 280 (2). pp. 272-277. ISSN 0003-2697
Full text not available from this repository.Abstract
We describe two near-infrared fluorescent squaraine dyes (Sq635 and Sq660), their spectra, their covalent linkage to proteins, and their use as donor and acceptor, respectively, in a fluorescence resonance energy transfer (FRET) immunoassay based on the use of red lasers. The dyes show quantum yields of around 10% in the free form and up to 68% when bound to proteins. If converted into their N-hydroxysuccinimide esters, they can be linked to free amino groups of proteins. To improve water solubility, two sulfo groups were introduced. The emission spectrum of Sq635 overlaps the absorption spectrum of Sq660, a fact that makes them a useful pair of dyes for use in FRET immunoassay which is demonstrated for human serum albumin/anti-human serum albumin. (C) 2000 Academic Press.
| Item Type: | Article |
|---|---|
| Uncontrolled Keywords: | SUCCINIMIDYL ESTERS; SPECTRAL PROPERTIES; LABELING REAGENTS; EXCITATION; |
| Subjects: | 500 Science > 540 Chemistry & allied sciences |
| Divisions: | Chemistry and Pharmacy > Institut für Analytische Chemie, Chemo- und Biosensorik > Chemo- und Biosensorik (Prof. Antje J. Bäumner, formerly Prof. Wolfbeis) |
| Depositing User: | Dr. Gernot Deinzer |
| Date Deposited: | 03 May 2022 13:44 |
| Last Modified: | 03 May 2022 13:44 |
| URI: | https://pred.uni-regensburg.de/id/eprint/42495 |
Actions (login required)
 |
View Item |
