Ruckstuhl, Thomas and Enderlein, Joerg and Jung, Stefan and Seeger, Stefan (2000) Forbidden light detection from single molecules. ANALYTICAL CHEMISTRY, 72 (9). pp. 2117-2123. ISSN 0003-2700
Full text not available from this repository. (Request a copy)Abstract
We present a new concept for ultrasensitive detection of surface-generated fluorescence which is made possible by a new optical module. The detection method leads to an enhancement in fluorescence collection efficiency to more than 65% of the total of emitted light, whereas high-aperture microscope objectives are able to collect 44% at best. Moreover, by employing this new optical module, the detection volume can be restricted to similar to 10(-17) L. This allows for an exceptional discrimination of bulk-generated against surface generated fluorescence, which may be of great value when surface-binding processes are monitored. We demonstrate the performance of the new detection system by detecting single fluorescent molecules and by determining antigen concentrations down to 5 fmol.
| Item Type: | Article |
|---|---|
| Uncontrolled Keywords: | PROTEIN MOLECULES; INTERFACE; FLUORESCENCE; SPECTROSCOPY; MICROSCOPY; EMISSION; SURFACE; |
| Subjects: | 500 Science > 540 Chemistry & allied sciences |
| Divisions: | Chemistry and Pharmacy > Institut für Analytische Chemie, Chemo- und Biosensorik |
| Depositing User: | Dr. Gernot Deinzer |
| Date Deposited: | 03 May 2022 13:53 |
| Last Modified: | 03 May 2022 13:53 |
| URI: | https://pred.uni-regensburg.de/id/eprint/42496 |
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