Stoelcker, Benjamin and Echtenacher, Bernd and Weich, Herbert A. and Sztajer, Helena and Hicklin, Daniel J. and Maennel, Daniela N. (2000) VEGF/Flk-1 interaction, a requirement for malignant ascites recurrence. JOURNAL OF INTERFERON AND CYTOKINE RESEARCH, 20 (5). pp. 511-517. ISSN 1079-9907, 1557-7465
Full text not available from this repository. (Request a copy)Abstract
Vascular endothelial growth factor (VEGF) plays an important role in the production of ascitic fluid associated with malignant tumor growth, In an experimental model for malignant ascites formation, mice were inoculated intraperitoneally with syngeneic mouse sarcoma tumor cells, Ascites development was not prevented by administering tumor necrosis factor (TNF) simultaneously with the tumor cell inoculation. When the malignant ascites was first drained and renewal of ascites was monitored, however, a TNF dose-dependent inhibition of ascitic fluid accumulation was observed. Northern blot analyses indicated transient downregulation by TNF on the expression of VEGF mRNA in tumor cells, Monoclonal antibody, (mAb) DC101 generated against the mouse VEGF receptor Flk-1 prevented the recurrence of malignant ascites in mice similar to TNF inhibition. In addition, exogenous soluble human Flt-1 used as an inhibitor of endogenous VEGF binding also inhibited ascites recurrence, These data demonstrate that the observed inhibitory effect of TNF on reestablishment of malignant ascites can be achieved equally by inhibition of the interaction of VEGF with its receptor Flk-1.
| Item Type: | Article |
|---|---|
| Uncontrolled Keywords: | ENDOTHELIAL GROWTH-FACTOR; TUMOR-NECROSIS-FACTOR; VASCULAR-PERMEABILITY FACTOR; INTERCELLULAR-ADHESION MOLECULE-1; EXPERIMENTAL LIVER METASTASIS; FACTOR-ALPHA; CELL GROWTH; FACTOR RECEPTOR; IN-VIVO; ANGIOGENESIS; |
| Subjects: | 600 Technology > 610 Medical sciences Medicine |
| Divisions: | Medicine > Lehrstuhl für Pathologie |
| Depositing User: | Dr. Gernot Deinzer |
| Date Deposited: | 10 May 2022 09:33 |
| Last Modified: | 10 May 2022 09:33 |
| URI: | https://pred.uni-regensburg.de/id/eprint/42531 |
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