A theoretical investigation of single-molecule fluorescence detection on thin metallic layers

Enderlein, Joerg (2000) A theoretical investigation of single-molecule fluorescence detection on thin metallic layers. BIOPHYSICAL JOURNAL, 78 (4). pp. 2151-2158. ISSN 0006-3495

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Abstract

In the present paper, the excitation and detection of single-molecule fluorescence over thin metallic films is studied theoretically within the framework of classical electrodynamics. The model takes into account the specific conditions of surface plasmon-assisted optical excitation, fluorescence quenching by the metal film, and detection geometry. Extensive numerical results are presented for gold, silver, and aluminum films, showing the detectable fluorescence intensities and their dependence on film thickness and the fluorescent molecule's position under optimal excitation conditions.

Item Type: Article
Uncontrolled Keywords: SURFACE;
Subjects: 500 Science > 540 Chemistry & allied sciences
Divisions: Chemistry and Pharmacy > Institut für Analytische Chemie, Chemo- und Biosensorik
Depositing User: Dr. Gernot Deinzer
Date Deposited: 17 May 2022 06:38
Last Modified: 17 May 2022 06:38
URI: https://pred.uni-regensburg.de/id/eprint/42606

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