Kotsianos, Dorothea and Bach, Daniel and Gamarra, Fernando and Haimerl, Wolfgang and Knuechel, Ruth and Lang, Susanne M. and Pfeifer, Klaus-Jürgen and Huber, Rudolf M. (2000) High-dose-rate brachytherapy: Dose escalation in three-dimensional miniorgans of the human bronchial wall. INTERNATIONAL JOURNAL OF RADIATION ONCOLOGY BIOLOGY PHYSICS, 46 (5). pp. 1267-1273. ISSN 0360-3016
Full text not available from this repository.Abstract
Purpose: High-dose-rate (HDR) brachytherapy of human lung cancer is well established, however fractionation schemes and dosages are based mainly on experience, The aim of this investigation was to study the effects of different doses of HDR iridium-192 on normal human bronchial epithelium in three-dimensional miniorgans of the human bronchial wall. Methods and Materials: Forty-eight biopsies from normal bronchi were cultivated for 14 days and exposed at random to different doses of HDR iridium 192 (0 Gy, 30 Gy, 45 Gy, 60 Gy, or 75 Gy). Cell viability was assessed immediately after irradiation, after 4 or 18 days by fluorescent staining, and cell damage of the culture was analyzed by light microscopy, Lactate dehydrogenase (LDH) was measured in the supernatant for 4 days. Results: There was no histologically apparent tissue damage regardless of the irradiation dose, The number of nonvital cells increased in irradiated miniorgans depending on the dose used (p < 0.05 at 75 Gy), This effect occur-red early and was less pronounced with time, LDH measurements showed an increase only in the first 24 hours. Conclusions: Our results confirm that normal bronchial epithelium has a high tolerance to early epithelial damage by irradiation. This model of human bronchial miniorgans is useful for further studies of the effects of irradiation on human bronchi, (C) 2000 Elsevier Science Inc.
| Item Type: | Article |
|---|---|
| Uncontrolled Keywords: | REMOTE AFTERLOADING BRACHYTHERAPY; STATIONARY ORGAN-CULTURE; RESPIRATORY EPITHELIUM; LUNG-CANCER; CARCINOMA; CELLS; SYSTEM; MUCOSA; lung cancer; radiotherapy; brachytherapy; miniorgan cultures |
| Subjects: | 600 Technology > 610 Medical sciences Medicine |
| Divisions: | Medicine > Lehrstuhl für Pathologie |
| Depositing User: | Dr. Gernot Deinzer |
| Date Deposited: | 24 May 2022 09:55 |
| Last Modified: | 24 May 2022 09:55 |
| URI: | https://pred.uni-regensburg.de/id/eprint/42740 |
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