Hans, Wolfgang and Schoelmerich, Juergen and Gross, Volker and Falk, Werner (2000) The role of the resident intestinal flora in acute and chronic dextran sulfate sodium-induced colitis in mice. EUROPEAN JOURNAL OF GASTROENTEROLOGY & HEPATOLOGY, 12 (3). pp. 267-273. ISSN 0954-691X
Full text not available from this repository. (Request a copy)Abstract
Objective There is increasing evidence that the intestinal microflora plays an important role in the pathogenesis of inflammatory bowel disease. In the present study, we examined the role of the resident intestinal flora in our model of dextran sulfate sodium (DSS)-induced acute and chronic colitis in mice. Methods Acute colitis was induced in BALB/c mice with 5% DSS in their drinking water for 7 days. Chronic colitis was established after four cycles of feeding 5% DSS for 7 days and water for 10 days. For eliminating intestinal bacteria, mice were injected intraperitoneally with metronidazole end ciprofloxacin. We analysed four parameters: (1) body weight, (2) length of the colon, (3) histological score, and (4) myeloperoxidase activity. Results In acute DSS colitis treatment with antibiotics led to an improvement of the histological parameters (epithelial damage, P< 0.05; inflammatory infiltrate, P< 0.05) and colon length (P< 0.0028). A significant reduction in granulocyte infiltration was indicated by a 52.6% reduced myeloperoxidase activity in colonic biopsies. By contrast, in chronic colitis, treatment of mice with antibiotics failed to show significant effects. Conclusion In acute DSS-induced colitis bacteria and/or bacterial products play a major role in initiation of inflammation but not in chronic DSS colitis. Eur J Gastroenterol Hepatol 12:267-273 (C) 2000 Lippincott Williams & Wilkins.
| Item Type: | Article |
|---|---|
| Uncontrolled Keywords: | INFLAMMATORY BOWEL-DISEASE; CHRONIC ULCERATIVE-COLITIS; TUMOR-NECROSIS-FACTOR; HELICOBACTER-HEPATICUS; INTERLEUKIN-10-DEFICIENT MICE; CONTROLLED TRIAL; C3H/HEJBIR MICE; BACTERIAL-FLORA; TRANSGENIC RATS; MURINE COLITIS; antibiotics; dextran sulfate sodium; experimental colitis; intestinal flora |
| Subjects: | 600 Technology > 610 Medical sciences Medicine |
| Divisions: | Medicine > Lehrstuhl für Innere Medizin I |
| Depositing User: | Dr. Gernot Deinzer |
| Date Deposited: | 24 May 2022 13:51 |
| Last Modified: | 24 May 2022 13:51 |
| URI: | https://pred.uni-regensburg.de/id/eprint/42777 |
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