Wagner, Charlotte and Goedecke, Axel and Ford, Michele and Schnermann, Juergen and Schrader, Juergen and Kurtz, Armin (2000) Regulation of renin gene expression in kidneys of eNOS-and nNOS-deficient mice. PFLUGERS ARCHIV-EUROPEAN JOURNAL OF PHYSIOLOGY, 439 (5). pp. 567-572. ISSN 0031-6768
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Our study aimed to assess the roles of nitric oxide derived from endothelium NO-synthase (eNOS) and macula densa neuronal NO-synthase (nNOS) in the regulation of renal renin expression. For this purpose renin mRNA levels and renin content were determined in kidneys of wild-type (wt), nNOS-deficient (nNOS-/-), and eNOS-deficient (eNOS-/-) mice, in which the renin system was suppressed by feeding a high-salt diet (NaCl 4%), or was stimulated by feeding a low-salt (NaCl 0.02%) diet together with the converting-enzyme inhibitor ramipril (10 mg kg(-1) day(-1)). In all mouse strains, renin mRNA levels were inversely related to the rate of sodium intake. In eNOS-/- mice renin mRNA levels and renal renin content were 50% lower than in wt mice at each level of salt intake, whilst in nNOS-/- mice renin expression was not different from wt controls. Administration of the general NO-synthase inhibitor nitro-L-arginine methyl ester (L-NAME, 50 mg kg(-1) day(-1)) to mice kept on the low-salt/ramipril regimen caused a decrease of renal renin mRNA levels in wt and nNOS-/- mice, but not in eNOS-/- mice. These observations suggest that neither eNOS nor nNOS is essential for up- or downregulation of renin expression. eNOS-derived NO appears to enhance renin expression, whereas nNOS-derived NO does not.
| Item Type: | Article |
|---|---|
| Uncontrolled Keywords: | NITRIC-OXIDE-SYNTHASE; KNOCKOUT MICE; MACULA DENSA; NO SYNTHASE; RELAXING FACTOR; DIETARY SALT; BLOCKADE; PRESSURE; RELEASE; HYPERTENSION; mRNA; NO synthase inhibition; NO synthase knockout; renin; renin content |
| Subjects: | 500 Science > 570 Life sciences |
| Divisions: | Biology, Preclinical Medicine > Institut für Physiologie > Prof. Dr. Armin Kurtz |
| Depositing User: | Dr. Gernot Deinzer |
| Date Deposited: | 31 May 2022 14:53 |
| Last Modified: | 31 May 2022 14:53 |
| URI: | https://pred.uni-regensburg.de/id/eprint/42802 |
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