Burghardt, Tillmann and Hochapfel, Florian and Salecker, Benjamin and Meese, Christine and Groene, Hermann-Josef and Rachel, Reinhard and Wanner, Gerhard and Krahn, Michael P. and Witzgall, Ralph (2015) Advanced electron microscopic techniques provide a deeper insight into the peculiar features of podocytes. AMERICAN JOURNAL OF PHYSIOLOGY-RENAL PHYSIOLOGY, 309 (12). F1082-F1089. ISSN 1931-857X, 1522-1466
Full text not available from this repository. (Request a copy)Abstract
Podocytes constitute the outer layer of the glomerular filtration barrier, where they form an intricate network of interdigitating foot processes which are connected by slit diaphragms. A hitherto unanswered puzzle concerns the question of whether slit diaphragms are established between foot processes of the same podocyte or between foot processes of different podocytes. By employing focused ion beam-scanning electron microscopy (FIB-SEM), we provide unequivocal evidence that slit diaphragms are formed between foot processes of different podocytes. We extended our investigations of the filtration slit by using dual-axis electron tomography of human and mouse podocytes as well as of Drosophila melanogaster nephrocytes. Using this technique, we not only find a single slit diaphragm which spans the filtration slit around the whole periphery of the foot processes but additional punctate filamentous contacts between adjacent foot processes. Future work will be necessary to determine the proteins constituting the two types of cell-cell contacts.
| Item Type: | Article |
|---|---|
| Uncontrolled Keywords: | FILTRATION; VISUALIZATION; PROTEINURIA; NEPHRIN; IMAGE; podocyte |
| Subjects: | 500 Science > 570 Life sciences |
| Divisions: | Biology, Preclinical Medicine > Institut für Biochemie, Genetik und Mikrobiologie > Lehrstuhl für Mikrobiologie > Prof. Dr. Reinhard Rachel Biology, Preclinical Medicine > Institut für Anatomie > Lehrstuhl für Molekulare und zelluläre Anatomie > Prof. Dr. Ralph Witzgall |
| Depositing User: | Dr. Gernot Deinzer |
| Date Deposited: | 29 Apr 2019 07:57 |
| Last Modified: | 29 Apr 2019 07:57 |
| URI: | https://pred.uni-regensburg.de/id/eprint/4282 |
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