Kraetzer, Friedrich and Rosorius, Olaf and Heger, Peter and Hirschmann, Nicole and Dobner, Thomas and Hauber, Joachim and Stauber, Roland H. (2000) The adenovirus type 5 E1B-55K oncoprotein is a highly active shuttle protein and shuttling is independent of E4orf6, p53 and Mdm2. ONCOGENE, 19 (7). pp. 850-857. ISSN 0950-9232
Full text not available from this repository.Abstract
The E1B-55K and E4orf6 oncoproteins of adenovirus type 5 are involved in the export of viral mRNAs, Previously, it cr as suggested that a complex composed of E1B-55K and E4orf6 serves as a nucleocytoplasmic transporter for viral mRNAs in which the E4orf6 protein directs both nuclear import and export, We now demonstrate that the E1B-55K protein itself shuttles efficiently in the absence of E4orf6, In addition, E1B-55K trafficking was independent of the defined shuttle proteins Mdm2 or p53, which interacts with E1B-55K, The identified N-terminal E1B-55K leucine-rich nuclear-export signal (NES) conferred rapid nuclear export even in a heterologous system in contrast to the postulated E4orf6NES, Interestingly, although shuttling was blocked by inhibitors of the CRM1 mediated export pathway, E1B-55K inhibited neither the activity nor the trafficking of the retroviral shuttle proteins HIV-1 Rev and HTLV-1 Rex, In contrast, Rev or Rex blocked the nuclear export of E1B-55K, most likely by competing for essential export factors, Our results provide new insights into the regulation of the adenovirus mRNA export system and the processes of adenovirus mediated transformation.
| Item Type: | Article |
|---|---|
| Uncontrolled Keywords: | REGION-4 34-KILODALTON PROTEIN; NUCLEAR EXPORT; 55-KILODALTON PROTEIN; MESSENGER-RNAS; INFECTED-CELLS; TRANSFORMED-CELLS; TUMOR-ANTIGEN; LIVING CELLS; REV PROTEIN; SEQUENCE; adenovirus; nucleo-cytoplasmic transport; oncoproteins; GFP |
| Subjects: | 600 Technology > 610 Medical sciences Medicine |
| Divisions: | Medicine > Lehrstuhl für Medizinische Mikrobiologie und Hygiene |
| Depositing User: | Dr. Gernot Deinzer |
| Date Deposited: | 07 Jun 2022 06:18 |
| Last Modified: | 07 Jun 2022 06:18 |
| URI: | https://pred.uni-regensburg.de/id/eprint/42821 |
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