Ammon, Christoph and Mondal, Krishna and Andreesen, Reinhard and Krause, Stefan W. (2000) Differential expression of the transcription factor NF-kappa B during human mononuclear phagocyte differentiation to macrophages and dendritic cells. BIOCHEMICAL AND BIOPHYSICAL RESEARCH COMMUNICATIONS, 268 (1). pp. 99-105. ISSN 0006-291X
Full text not available from this repository.Abstract
An important role for the Rel/NF-kappa B family of transcription factors in the differentiation process of dendritic cells (DC) and macrophages (MAC) was recently suggested by a number of mouse knockout studies but only little information is available for defined populations of human cells. To investigate the role of individual NF-KB proteins [p50, p52, p65 (RelA), RelB] in the differentiation of monocyte-derived cell types we analyzed and compared the expression pattern and DNA binding activity of NF-kappa B members in human monocytes (MO), MO-derived MAC, and MO-derived DC. Constitutive expression of p65 and RelB mRNA was found in MO and no significant regulation was observed during differentiation of MO into MAC or immature DC. Only during lipopolysaccharide-induced terminal differentiation of DC was a marked increase in RelB mRNA detected. In DNA binding assays performed with nuclear extracts from blood MO, p50/p50 homodimers were mainly detected, whereas complexes containing p50/RelB and p50/p65 heterodimers were less abundant. DNA-bound protein complexes containing p50/RelB and p50/p65 increased and additional p65/p65 complexes appeared during differentiation of MO into either MAC or immature DC. A strong increase in complexes containing p50/RElB was observed during terminal differentiation of DC. Therefore, gradual differences in the DNA binding activities of different NF-kappa B homo- and heterodimers correlate with differentiation stages of MO, MAC, and DC and are probably important for the biological role of these cells. (C) 2000 Academic Press.
| Item Type: | Article |
|---|---|
| Uncontrolled Keywords: | TARGETED DISRUPTION; MONOCYTIC CELLS; MESSENGER-RNA; HUMAN BLOOD; ACTIVATION; LIPOPOLYSACCHARIDE; SUBUNIT; ALPHA; GENE; MATURATION; monocyte; macrophage; dendritic cell; NF-kappa B; RelB |
| Subjects: | 600 Technology > 610 Medical sciences Medicine |
| Divisions: | Medicine > Lehrstuhl für Innere Medizin III (Hämatologie und Internistische Onkologie) |
| Depositing User: | Dr. Gernot Deinzer |
| Date Deposited: | 01 Jun 2022 06:03 |
| Last Modified: | 01 Jun 2022 06:03 |
| URI: | https://pred.uni-regensburg.de/id/eprint/42829 |
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