Dirmeier, R. and Hauska, Guenther and Stetter, Karl Otto (2000) ATP synthesis at 100 degrees C by an ATPase purified from the hyperthermophilic archaeon Pyrodictium abyssi. FEBS LETTERS, 467 (1). pp. 101-104. ISSN 0014-5793
Full text not available from this repository. (Request a copy)Abstract
The chemolithoautotrophic archaeon Pyrodictium abyssi isolate TAG 11 lives close to 100 degrees C and gains energy by sulfur respiration, with hydrogen as electron donor. From the membranes of this hyperthermophile, an ATPase complex was isolated. The purified enzyme consists of six major polypeptides, the 67, 51, 41, 26 and 22 kDa subunits composing the AF(1) headpiece, and the 7 kDa proteolipid of the AF(0) component. The headpiece of the enzyme restored the formation of ATP during sulfur respiration in membrane vesicles from which it had been removed by low salt treatment. Characteristics of the reconstituted activity suggest that the same enzyme is responsible for ATP formation in untreated membranes. ATP formation was neither sensitive to ionophores and uncouplers, nor to dicyclohexyl carbodiimide, but depended on closed vesicles. Both ATPase activity (up to 2 mu mol per min and mg protein) as well as ATP formation (up to 0.4 mu mol per min and mg membrane protein) were highest at 100 degrees C. A P/e2 ratio of close to one can be estimated for sulfur respiration with hydrogen. In addition to ATP, autoradiographic detection revealed the formation of high quantities of P-33(i)-labeled ADP and of another compound not identified so far. (C) 2000 Federation of European Biochemical Societies.
| Item Type: | Article |
|---|---|
| Uncontrolled Keywords: | SULFOLOBUS-ACIDOCALDARIUS; H+-ATPASES; ACIDOTHERMOPHILIC ARCHAEBACTERIUM; PURIFICATION; VACUOLAR; SYNTHASE; MEMBRANES; SEQUENCES; EVOLUTION; HALOBIUM; hyperthermophile; archaeon; ATPase; ATP synthesis; Pyrodictium |
| Subjects: | 500 Science > 580 Botanical sciences |
| Divisions: | Biology, Preclinical Medicine > Institut für Pflanzenwissenschaften > Alumni or Retired > Prof. Dr. Günter Hauska |
| Depositing User: | Dr. Gernot Deinzer |
| Date Deposited: | 07 Jun 2022 06:43 |
| Last Modified: | 07 Jun 2022 06:43 |
| URI: | https://pred.uni-regensburg.de/id/eprint/42831 |
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