Roth, Christina and Schuierer, Marion and Guenther, Kalle and Buettner, Reinhard (2000) Genomic structure and DNA binding properties of the human zinc finger transcriptional repressor AP-2rep (KLF12). GENOMICS, 63 (3). pp. 384-390. ISSN 0888-7543
Full text not available from this repository. (Request a copy)Abstract
We recently cloned a novel murine transcriptional repressor, the Kruppel-like zinc finger protein AP-2rep (HGMW-approved symbol KLF12), that binds to a regulatory element in the AP-2 alpha gene promoter. In the present study, we characterize the human AP-2rep homolog and describe expression patterns in human urogenital and lymphoma cell lines. The predicted human protein of 402 amino acids exhibits 95.8% identity and 98.5% similarity to the murine AP-2rep peptide. The genomic locus of human AP-2rep consists of seven exons and was assigned to chromosome 13q22 by fluorescence in situ hybridization to metaphase chromosomes. Human AP-2rep repressed both reporter expression from a transiently transfected AP-2 alpha promoter and the endogenous AP-2 alpha gene and inversely was negatively regulated by AP-2 alpha. The consensus motif CAGTGGG was identified by an in vitro binding site selection assay. In summary, our data further point to an important role of AP-2rep as a transcriptional silencer and reveal reciprocal regulation of AP-2 alpha and AP-2rep. (C) 2000 Academic Press.
| Item Type: | Article |
|---|---|
| Uncontrolled Keywords: | MOUSE EMBRYOGENESIS; GENE-EXPRESSION; DOWN-REGULATION; AP-2-BETA; CLONING; ACTIVATION; CARCINOMA; PROTEINS; MELANOMA; AP-2.2; |
| Subjects: | 600 Technology > 610 Medical sciences Medicine |
| Divisions: | Medicine > Lehrstuhl für Pathologie |
| Depositing User: | Dr. Gernot Deinzer |
| Date Deposited: | 07 Jun 2022 09:06 |
| Last Modified: | 07 Jun 2022 09:06 |
| URI: | https://pred.uni-regensburg.de/id/eprint/42852 |
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