Sprott, H and Muller-Ladner, U and Distler, O and Gay, RE and Barnum, SR and Landthaler, M and Scholmerich, J and Lang, B and Gay, S (2000) Detection of activated complement complex C5b-9 and complement receptor C5a in skin biopsies of patients with systemic sclerosis (scleroderma). JOURNAL OF RHEUMATOLOGY, 27 (2). pp. 402-404. ISSN 0315-162X,
Full text not available from this repository.Abstract
Objective. Upregulated matrix synthesis is a hallmark of systemic sclerosis (SSc), There are indications that growth factors such as platelet derived growth factor (PDGF) are involved in proliferative pathways in SSc lesions. As activated complement releases PDGF from endothelial cells, we searched for activated complement and the complement receptor for C5a (C5aR) in skin biopsies of patients with SSc. Methods. Snap frozen sections of 8 patients with early SSc and 5 patients with longterm SSc were examined. Using monoclonal antibodies against activated complement complex C5b-9 and the C5aR, skin biopsies derived from both clinically involved and non-involved skin were examined by APAAP immunohistochemistry. Results. A pattern of activated complement C5b-9 and the C5aR could be detected in SSc microvasculature. Eleven of the 13 patients (7/8 patients with early SSc) showed positive staining for C5b-9. The C5aR was detected in 6 of the 8 patients with early SSc. In 3 patients with longterm disease, C5aR expression could also be detected in non-involved skin. Conclusion. Activated complement and complement receptors could be detected in early and late stages of SSc skin lesions. The presence of complement receptors in non-involved skin may indicate preclinical activation of pathways resulting in growth factor dependent matrix synthesis.
| Item Type: | Article |
|---|---|
| Uncontrolled Keywords: | GROWTH-FACTOR; EXPRESSION; IMMUNOHISTOCHEMISTRY; COLLAGEN; PROTEIN; CELLS; scleroderma; complement; skin; platelet derived growth factor |
| Subjects: | 600 Technology > 610 Medical sciences Medicine |
| Divisions: | Medicine > Lehrstuhl für Dermatologie und Venerologie Medicine > Lehrstuhl für Innere Medizin I |
| Depositing User: | Dr. Gernot Deinzer |
| Date Deposited: | 21 May 2021 08:10 |
| Last Modified: | 21 May 2021 08:10 |
| URI: | https://pred.uni-regensburg.de/id/eprint/42860 |
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